Shotgun proteomic analyses of Pseudomonas species isolated from fish products

Ana G Abril; Pilar Calo-Mata; Karola Böhme; Tomás G Villa; Jorge Barros-Velázquez; Ángeles Sánchez-Pérez; Manuel Pazos; Mónica Carrera

doi:10.1016/j.foodchem.2024.139342

Shotgun proteomic analyses of Pseudomonas species isolated from fish products

Food Chem. 2024 Apr 12:450:139342. doi: 10.1016/j.foodchem.2024.139342. Online ahead of print.

Authors

Ana G Abril¹, Pilar Calo-Mata², Karola Böhme³, Tomás G Villa⁴, Jorge Barros-Velázquez⁵, Ángeles Sánchez-Pérez⁶, Manuel Pazos⁷, Mónica Carrera⁸

Affiliations

¹ Department of Food Technology, Marine Research Institute (IIM-CSIC), Spanish National Research Council (CSIC), 36208 Vigo, Spain; Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15898 Santiago de Compostela, Spain. Electronic address: agonzalez@iim.csic.es.
² Department of Analytical Chemistry, Nutrition and Food Science, Food Technology Division, School of Veterinary Sciences, University of Santiago de Compostela, Campus Lugo, 27002 Lugo, Spain. Electronic address: p.calo.mata@usc.es.
³ Department of Analytical Chemistry, Nutrition and Food Science, Food Technology Division, School of Veterinary Sciences, University of Santiago de Compostela, Campus Lugo, 27002 Lugo, Spain.
⁴ Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, 15898 Santiago de Compostela, Spain.
⁵ Department of Analytical Chemistry, Nutrition and Food Science, Food Technology Division, School of Veterinary Sciences, University of Santiago de Compostela, Campus Lugo, 27002 Lugo, Spain. Electronic address: jorge.barros@usc.es.
⁶ Sydney School of Veterinary Science, Faculty of Science, University of Sydney, NSW 2006, Australia.
⁷ Department of Food Technology, Marine Research Institute (IIM-CSIC), Spanish National Research Council (CSIC), 36208 Vigo, Spain. Electronic address: mpazos@iim.csic.es.
⁸ Department of Food Technology, Marine Research Institute (IIM-CSIC), Spanish National Research Council (CSIC), 36208 Vigo, Spain. Electronic address: mcarrera@iim.csic.es.

PMID: 38631198
DOI: 10.1016/j.foodchem.2024.139342

Abstract

Numerous Pseudomonas species can infect aquatic animals, such as farmed rainbow trout, sea trout, sea bass, and sea bream, by causing disease or stress reactions. In aquaculture facilities, a number of Pseudomonas species have been isolated and identified as the main pathogens. The present study describes the characterization of 18 Pseudomonas strains, isolated from fish products using shotgun proteomics. The bacterial proteomes obtained were further analyzed to identify the main functional pathway proteins involved. In addition, this study revealed the presence of 1015 non-redundant peptides related to virulence factors. An additional 25 species-specific peptides were identified as putative Pseudomonas spp. biomarkers. The results constitute the largest dataset, described thus far for the rapid identification and characterization of Pseudomonas species present in edible fish; furthermore, these data can provide the basis for further research into the development of new therapies against these harmful pathogens.

Keywords: Bacterial detection and identification; Discovery proteomics; Food; LC–ESI–MS/MS; Mass spectrometry; Pathogenic bacteria; Seafood.