Generation of a human induced pluripotent stem cell line (SHUPLi002-A) from PBMCs of a healthy female donor

Beixu Li; Xing Ye; Junyi Lin; Kaijun Ma; Meng He; Youxin Fang

doi:10.1016/j.scr.2024.103422

Generation of a human induced pluripotent stem cell line (SHUPLi002-A) from PBMCs of a healthy female donor

Stem Cell Res. 2024 Apr 15:77:103422. doi: 10.1016/j.scr.2024.103422. Online ahead of print.

Authors

Beixu Li¹, Xing Ye², Junyi Lin³, Kaijun Ma⁴, Meng He⁵, Youxin Fang⁶

Affiliations

¹ School of Policing Studies, Shanghai University of Political Science and Law, Shanghai, China; Shanghai Fenglin Forensic Center, Shanghai, China.
² Department of Forensic Medicine, School of Basic Medical Sciences, Gannan Medical University, Jiangxi, China.
³ Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China.
⁴ Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai, China.
⁵ Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, China. Electronic address: 042101055@fudan.edu.cn.
⁶ Department of Neurology, Minhang Hospital, Fudan University, Shanghai, China. Electronic address: youxin_fang@163.com.

PMID: 38631181
DOI: 10.1016/j.scr.2024.103422

Abstract

Human induced pluripotent stem cells (iPSCs) have good multi-lineage differentiation potential, which is an ideal model for studying the pathogenesis of diseases and drug screening.Here, we generated an iPSC line, SHUPLi002-A, from peripheral blood mononuclear cells (PBMCs) of a healthy 28-year-old female individual using episomal vectors. SHUPLi002-A is characterized by expression of classical pluripotent stem cell markers as well as teratoma formation assays to evaluate their differentiation capacity in vivo.