ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis

Liuqing Wu; Kaiqi Wang; Mengyi Chen; Wenxin Su; Zheng Liu; Xiaoying Guo; Mengqian Ma; Shuangjie Qian; Yuqi Deng; Haihan Wang; Chanjuan Mao; Zaibao Zhang; Xiaofeng Xu

doi:10.1007/s12298-024-01425-w

ALLENE OXIDE SYNTHASE (AOS) induces petal senescence through a novel JA-associated regulatory pathway in Arabidopsis

Physiol Mol Biol Plants. 2024 Feb;30(2):199-212. doi: 10.1007/s12298-024-01425-w. Epub 2024 Mar 8.

Authors

Liuqing Wu^#¹, Kaiqi Wang^#², Mengyi Chen¹, Wenxin Su¹, Zheng Liu¹, Xiaoying Guo¹, Mengqian Ma¹, Shuangjie Qian¹, Yuqi Deng¹, Haihan Wang³, Chanjuan Mao¹, Zaibao Zhang⁴, Xiaofeng Xu¹

Affiliations

¹ Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China.
² College of Biological and Environmental Engineering, Jingdezhen University, Jiangxi, 333000 China.
³ School of Biological Science, University of California Irvine, Irvine, USA.
⁴ School of Life and Health Science, Huzhou College, Huzhou, Zhejiang China.

^# Contributed equally.

PMID: 38623171
PMCID: PMC11016053 (available on 2025-02-01)
DOI: 10.1007/s12298-024-01425-w

Abstract

Flowers are crucial for the reproduction of flowering plants and their senescence has drastic effects on plant-animal interactions as well as pollination. Petal senescence is the final phase of flower development which is regulated by hormones and genes. Among these, jasmonic acid (JA) has emerged as a major contributor to petal senescence, but its molecular mechanisms remain elusive. Here, the role of JA in petal senescence in Arabidopsis was investigated. We showed that petal senescence in aos mutant was significantly delayed, which also affected petal cell size and proliferation. Similar significant delays in petal senescence were observed in dad1 and coi1 mutants. However, MYB21/24 and MYC2/3/4, known downstream regulators of JA in flower development, played no role in petal senescence. This indicated that JA regulates petal senescence by modulating other unknown transcription factors. Transcriptomic analysis revealed that AOS altered the expression of 3681 genes associated, and identified groups of differentially expressed transcription factors, highlighting the potential involvement of AP-2, WRKY and NAC. Furthermore, bHLH13, bHLH17 and URH2 were identified as potential new regulators of JA-mediated petal senescence. In conclusion, our findings suggest a novel genetic pathway through which JA regulates petal senescence in Arabidopsis. This pathway operates independently of stamen development and leaf senescence, suggesting the evolution of specialized mechanisms for petal senescence.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-024-01425-w.

Keywords: AOS; Arabidopsis thaliana; Jasmonic acid; Petal senescence.

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