Oligopeptide-based molecular labelling of (bio)degradable polyester biomaterials

Joanna Rydz; Khadar Duale; Wanda Sikorska; Marta Musioł; Henryk Janeczek; Andrzej Marcinkowski; Mariola Siwy; Grażyna Adamus; Przemysław Mielczarek; Jerzy Silberring; Jan Juszczyk; Ewa Piętka; Iza Radecka; Abhishek Gupta; Marek Kowalczuk

doi:10.1016/j.ijbiomac.2024.131561

Oligopeptide-based molecular labelling of (bio)degradable polyester biomaterials

Int J Biol Macromol. 2024 Apr 16;268(Pt 1):131561. doi: 10.1016/j.ijbiomac.2024.131561. Online ahead of print.

Authors

Affiliations

¹ Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Skłodowskiej 34, 41-800 Zabrze, Poland; Department of Food, Agricultural and Biological Engineering, The Ohio State University, Wooster 44691, OH, United States. Electronic address: jrydz@cmpw-pan.pl.
² Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Skłodowskiej 34, 41-800 Zabrze, Poland.
³ Department of Analytical Chemistry and Biochemistry, AGH University of Science and Technology, A. Mickiewicza 30, 30-059 Kraków, Poland; Laboratory of Proteomics and Mass Spectrometry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343 Kraków, Poland.
⁴ Department of Analytical Chemistry and Biochemistry, AGH University of Science and Technology, A. Mickiewicza 30, 30-059 Kraków, Poland.
⁵ Department of Medical Informatics and Artificial Intelligence, Faculty of Biomedical Engineering, Silesian University of Technology, Roosevelta 40, 41-800 Zabrze, Poland.
⁶ School of Life Sciences, Faculty of Science and Engineering, University of Wolverhampton, Wulfruna St., Wolverhampton WV1 1LY, UK.
⁷ School of Pharmacy, Faculty of Science and Engineering, University of Wolverhampton, City Campus, Wulfruna St., Wolverhampton WV1 1LY, UK.
⁸ Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Skłodowskiej 34, 41-800 Zabrze, Poland; School of Life Sciences, Faculty of Science and Engineering, University of Wolverhampton, Wulfruna St., Wolverhampton WV1 1LY, UK.

PMID: 38621562
DOI: 10.1016/j.ijbiomac.2024.131561

Abstract

Nowadays, a very important motivation for the development of new functional materials for medical purposes is not only their performance but also whether they are environmentally friendly. In recent years, there has been a growing interest in the possibility of labelling (bio)degradable polymers, in particular those intended for specific applications, especially in the medical sector, and the potential of information storage in such polymers, making it possible, for example, to track the ultimate environmental fate of plastics. This article presents a straightforward green approach that combines both aspects using an oligopeptide, which is an integral part of polymer material, to store binary information in a physical mixture of polymer and oligopeptide. In the proposed procedure the year of production of polymer films made of poly(l-lactide) (PLLA) and a blend of poly(1,4-butylene adipate-co-1,4-butylene terephthalate) and polylactide (PBAT/PLA) were encoded as the sequence of the appropriate amino acids in the oligopeptide (PEP) added to these polymers. The decoding of the recorded information was carried out using mass spectrometry technique as a new method of decoding, which enabled the successful retrieval and reading of the stored information. Furthermore, the properties of labelled (bio)degradable polymer films and stability during biodegradation of PLLA/PEP film under industrial composting conditions have been investigated. The labelled films exhibited good oligopeptide stability, allowing the recorded information to be retrieved from a green polymer/oligopeptide system before and after biodegradation. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay) study of the PLLA and PLLA/PBAT using the MRC-5 mammalian fibroblasts was presented for the first time.

Keywords: Identification; Labelling; Macromolecular information storage; Peptide; Poly(1,4-butylene adipate-co-1,4-butylene terephthalate); Polyester; Polylactide.