Peroxynitrite-activated fluorescent probe with two reaction triggers for pathological diagnosis and therapeutic evaluation of inflammation

Feiyan Liu; Manman Li; Weixia Li; Yingjie Ren; Mingliang Zhang; Hui Zhang; Pan Wang; Yali Wu; Kehan Wang; Xiaoyan Wang; Xiaofei Chen; Jinfa Tang

doi:10.1016/j.bioorg.2024.107362

Peroxynitrite-activated fluorescent probe with two reaction triggers for pathological diagnosis and therapeutic evaluation of inflammation

Bioorg Chem. 2024 Apr 9:147:107362. doi: 10.1016/j.bioorg.2024.107362. Online ahead of print.

Authors

Feiyan Liu¹, Manman Li¹, Weixia Li², Yingjie Ren¹, Mingliang Zhang¹, Hui Zhang¹, Pan Wang¹, Yali Wu¹, Kehan Wang¹, Xiaoyan Wang¹, Xiaofei Chen³, Jinfa Tang⁴

Affiliations

¹ Henan Province Engineering Research Center for Clinical Application, Evaluation and Transformation of Traditional Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, Henan Province Engineering Research Center of Safety Evaluation and Risk Management of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine, Henan, Zhengzhou 450000, China.
² Henan Province Engineering Research Center for Clinical Application, Evaluation and Transformation of Traditional Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, Henan Province Engineering Research Center of Safety Evaluation and Risk Management of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine, Henan, Zhengzhou 450000, China; Henan University of Chinese Medicine, Henan, Zhengzhou 450000, China. Electronic address: liweixia01@hactcm.edu.cn.
³ Henan Province Engineering Research Center for Clinical Application, Evaluation and Transformation of Traditional Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, Henan Province Engineering Research Center of Safety Evaluation and Risk Management of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine, Henan, Zhengzhou 450000, China. Electronic address: chenxiaofei5555@163.com.
⁴ Henan Province Engineering Research Center for Clinical Application, Evaluation and Transformation of Traditional Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, Henan Province Engineering Research Center of Safety Evaluation and Risk Management of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine, Henan, Zhengzhou 450000, China; Henan University of Chinese Medicine, Henan, Zhengzhou 450000, China. Electronic address: tangjinfa@hactcm.edu.cn.

PMID: 38615474
DOI: 10.1016/j.bioorg.2024.107362

Abstract

Excessive peroxynitrite (ONOO^-) is closely related to the occurrence and progression of inflammation. Therefore, the development of an efficacious ONOO^- activatable probe holds great potential for the early diagnosis of pathological inflammation, and the direct evaluation of the therapeutic efficacy of active protectants. In this work, a new ONOO^--activated fluorescent probe (SZP) which greatly improved the specificity and sensitivity (LOD = 8.03 nM) with large Stokes shift (150 nm) through introducing two reaction triggers (diphenyl phosphinate moiety, CC unsaturated bond) was rationally designed for rapid detecting ONOO^- (within 2 min). The excellent properties of probe SZP enable it to realize the fluorescence-guided diagnosis of inflammation. More importantly, probe SZP has also been utilized to assess the anti-inflammatory efficacy of traditional Chinese medicines (TCMs) active ingredients for the remediation of inflammation by monitoring ONOO^- fluctuation for the first time.

Keywords: Fluorescent probe; Inflammation; Peroxynitrite; TCMs ingredients; Two reaction triggers.