Polyvinylpyrrolidone can prevent oolemma lysis caused by abnormal rupture of the plasma membrane in Piezo-ICSI

Kohei Mizuno; Osamu Okitsu; Mako Goto; Atsuko Kusuhara; Koji Kusuhara

doi:10.1007/s13577-024-01061-2

Polyvinylpyrrolidone can prevent oolemma lysis caused by abnormal rupture of the plasma membrane in Piezo-ICSI

Hum Cell. 2024 Apr 14. doi: 10.1007/s13577-024-01061-2. Online ahead of print.

Authors

Kohei Mizuno¹, Osamu Okitsu², Mako Goto², Atsuko Kusuhara², Koji Kusuhara²

Affiliations

¹ Kusuhara Women's Clinic, Chuo-Ku, Tokyo, Japan. kusuhara.labo@gmail.com.
² Kusuhara Women's Clinic, Chuo-Ku, Tokyo, Japan.

PMID: 38615310
DOI: 10.1007/s13577-024-01061-2

Abstract

The aim of this study was to investigate whether it is possible to prevent oolemma lysis after Piezo-assisted intracytoplasmic sperm injection (Piezo-ICSI) caused by abnormal membrane rupture. A total of 489 mature oocytes were obtained from 85 patients who underwent Piezo-ICSI in an infertility clinic. Inseminated oocytes were classified into the following two groups: normal rupture and abnormal rupture, and a portion of abnormally ruptured oocytes was randomly exposed to 7% PVP solution. Normal fertilization rate, degeneration rate, proportion of high-quality embryos on day 3, blastocyst formation, and morphologically high-quality blastocysts were compared. Abnormal rupture was defined as cytoplasmic membrane rupture before piezo pulse driving. Among the abnormal rupture groups, the normal fertilization and degeneration rates were compared between the high-stretched (ruptured at ≥ 50% during oocyte membrane stretching) and low-stretched (< 50% position) oocytes.The degeneration rate was significantly higher in abnormally ruptured oocytes than in normally ruptures oocytes (14.3% vs 1.3%, p < 0.001), and there was no significant difference in embryonic development after fertilization. PVP treatment immediately after oolemma rupture significantly decreased the degeneration rate (6.0% vs 19.7%, p = 0.031) and increased the normal fertilization rate. Similarly, there were no significant differences in the developmental potential. Furthermore, pregnancy outcome data showed no significant differences in pregnancy and live birth rates. The degeneration rate was reduced by treating low-stretched oocytes with PVP.Exposure to polyvinylpyrrolidone (PVP) immediately after abnormal membrane rupture represents an effective strategy to prevent oocyte degeneration. This is the first study to propose a strategy for the prevention of oocyte degeneration in Piezo-ICSI. These findings are expected to reduce the oocyte degeneration rate and increase normal fertilization rate as well as assist patients who can only acquire oocytes with weak plasma membranes.

Keywords: Abnormal rupture; Line tension; Piezo-assisted intracytoplasmic sperm injection; Plasma membrane tension; Polyvinylpyrrolidone.