A novel fluorescent probe based imprinted polymer-coated magnetite for the detection of imatinib leukemia anti-cancer drug traces in human plasma samples

Heba M Hashem; Eslam A Ghaith; Amira Eladl; Samira M Abozeid; A B Abdallah

doi:10.1016/j.saa.2024.124262

A novel fluorescent probe based imprinted polymer-coated magnetite for the detection of imatinib leukemia anti-cancer drug traces in human plasma samples

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Apr 10:315:124262. doi: 10.1016/j.saa.2024.124262. Online ahead of print.

Authors

Heba M Hashem¹, Eslam A Ghaith², Amira Eladl³, Samira M Abozeid², A B Abdallah²

Affiliations

¹ Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt. Electronic address: hebamaher89@mans.edu.eg.
² Chemistry Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
³ Pharmacology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.

PMID: 38613900
DOI: 10.1016/j.saa.2024.124262

Abstract

Myeloid leukemia is a chronic cancer, which associated with abnormal BCR-ABL tyrosine kinase activity. Imatinib (IMB) acts as a tyrosine kinase inhibitor and averts tumor growth in cancer cells by controlling cell division, so it is urgent to develop an effective assay to detect and monitor its IMB concentration. Therefore, an innovative fluorescent biomimetic sensor is a promising sensing material that constructed for the efficient recognition of IMB and displays excellent selectivity and sensitivity stemming from molecularly imprinted polymer@Fe₃O₄ (MIP@Fe₃O₄). The detection strategy depends on the recognition of IMB molecules at the imprinted sites in the presence of coexisting molecules, which are then transferred to the fluorescence signal. The synthesized MIP@Fe₃O₄ was characterized using Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and atomic force microscopy (AFM). Furthermore, computational studies of the band gap (E_HOMO-E_LUMO) of the monomers, IMB, and their complexes were performed. These results confirmed that the copolymer is the most appropriate and has high stability (Binding energy; 0.004 x 10^-19 KJ) and low reactivity. A comprehensive linear response over IMB concentrations from 5 × 10^-6 mol/L to 8 × 10^-4 mol/L with a low detection limit of 9.3 × 10^-7 mol/L was achieved. Furthermore, the proposed technique displayed long-term stability (over 2 months), high intermediate precision (RSD<2.1 %), good reproducibility (RSD <1.9 %), and outstanding selectivity toward IMB over analogous molecules with similar chemical and spatial structure (no interference by 100 to 150-fold of the competitors). Owing to these merits, the proposed fluorescence sensor was utilized to detect IMB in drug tablets and human plasma, and satisfactory results (99.3-100.4 %) were obtained. Thus, the synthesized fluorescence sensor is a promising platform for IMB sensing in various applications.

Keywords: Blood sample; Fluorescence sensor; Imatinib; Imprinted polymer; Leukemia cancer.