Exploration of Exopolysaccharide from Leuconostoc mesenteroides HDE-8: Unveiling Structure, Bioactivity, and Food Industry Applications

Yi Yang; Guangbin Ye; Xintong Qi; Bosen Zhou; Liansheng Yu; Gang Song; Renpeng Du

doi:10.3390/polym16070954

Exploration of Exopolysaccharide from Leuconostoc mesenteroides HDE-8: Unveiling Structure, Bioactivity, and Food Industry Applications

Polymers (Basel). 2024 Mar 30;16(7):954. doi: 10.3390/polym16070954.

Authors

Yi Yang¹, Guangbin Ye², Xintong Qi¹, Bosen Zhou¹, Liansheng Yu¹, Gang Song^{1

3}, Renpeng Du^{1

3

4}

Affiliations

¹ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
² Institute of Life Sciences, Youjiang Medical University for Nationalities, Baise 533000, China.
³ Hebei Key Laboratory of Agroecological Safety, Hebei University of Environmental Engineering, Qinhuangdao 066102, China.
⁴ State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Abstract

A strain of Leuconostoc mesenteroides HDE-8 was isolated from homemade longan fermentation broth. The exopolysaccharide (EPS) yield of the strain was 25.1 g/L. The EPS was isolated and purified, and the structure was characterized using various techniques, including X-ray diffraction (XRD), nuclear magnetic resonance (NMR) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, high-performance size exclusion chromatography (HPSEC), and scanning electron microscopy (SEM). The monosaccharide composition of the EPS was glucose, with a molecular weight (Mw) of 1.7 × 10⁶ Da. NMR spectroscopy revealed that the composition of the HDE-8 EPS consisted of D-glucose pyranose linked by α-(1→4) and α-(1→6) bonds. The SEM analysis of the EPS showed an irregular sheet-like structure. Physicochemical analysis demonstrated that EPSs exhibit excellent thermal stability and high viscosity, making them suitable for fermentation in heat-processed and acidic foods. Additionally, milk coagulation tests showed that the presence of EPSs promotes milk coagulation when supplemented with sucrose. It suggests that EPSs have wide-ranging potential applications as food additives, improving the texture and taste of dairy products. This study provides practical guidance for the commercial use of HDE-8 EPSs in the food and related industries.

Keywords: Leuconostoc mesenteroides; exopolysaccharide; food application; physicochemical; structure.

Grants and funding

The Outstanding Youth Fund Project of the Natural Science Foundation of the Heilongjiang Province [No. YQ2021C030] (Renpeng Du). A project funded by the China Postdoctoral Science Foundation [No. 2022MD713755] (Renpeng Du). The Heilongjiang Province Postdoctoral Funding Project [No. LBH-Z21082] (Renpeng Du). The Open Funding Project of the State Key Laboratory of Microbial Metabolism [No. MMLKF22-07] (Renpeng Du). The Heilongjiang Province provincial colleges and universities basic scientific research business expenses scientific research projects [No. 2022-KYYWF-1075] (Renpeng Du).