Inhalation of 2, 4-di-tert-butylphenol-Loaded micelles suppresses respiratory syncytial virus infection in mice

Mingyang Guo; Jianqing Peng; Pengcheng Guo; Qin Wang; Lin Zhang; Huyan Shen; Fang Chen; Pingping Zhang; Siyu Lin; Han Gao; Hong Peng; Rong Mou; Jiandong Huang; Jianxin Wang; Yu-Si Luo; Ke Zhang

doi:10.1016/j.antiviral.2024.105880

Inhalation of 2, 4-di-tert-butylphenol-Loaded micelles suppresses respiratory syncytial virus infection in mice

Antiviral Res. 2024 Apr 10:226:105880. doi: 10.1016/j.antiviral.2024.105880. Online ahead of print.

Authors

Mingyang Guo¹, Jianqing Peng², Pengcheng Guo³, Qin Wang², Lin Zhang¹, Huyan Shen¹, Fang Chen¹, Pingping Zhang¹, Siyu Lin¹, Han Gao⁴, Hong Peng⁴, Rong Mou¹, Jiandong Huang⁵, Jianxin Wang³, Yu-Si Luo⁶, Ke Zhang⁷

Affiliations

¹ Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province / Virology Institute / The Key and Characteristic Laboratory of Modern Pathogenicity Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
² The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, The Key Laboratory of Optimal Utilization of Natural Medicine Resources, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 561113, China.
³ Department of Pharmaceutics, School of Pharmacy, Ministry of Education, Fudan University & Key Laboratory of Smart Drug Delivery, Shanghai 201203, China.
⁴ Department of Emergency ICU, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China.
⁵ School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Clinical Oncology Center, Shenzhen Key Laboratory for Cancer Metastasis and Personalized Therapy, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518000, China.
⁶ Department of Emergency ICU, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China; Department of Emergency, Liupanshui Hospital of The Affiliated Hospital of Guizhou Medical University, Liupanshui 553000, China. Electronic address: roseluogy@outlook.com.
⁷ Key Laboratory of Microbio and Infectious Disease Prevention & Control in Guizhou Province / Virology Institute / The Key and Characteristic Laboratory of Modern Pathogenicity Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, The Key Laboratory of Optimal Utilization of Natural Medicine Resources, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 561113, China. Electronic address: kezhang@gmc.edu.cn.

PMID: 38608838
DOI: 10.1016/j.antiviral.2024.105880

Abstract

Human respiratory syncytial virus (RSV) is a common cause of respiratory infections in infants, young children, and elderly people. However, there are no effective treatments or vaccines available in most countries. In this study, we explored the anti-RSV potential of 2, 4-Di-tert-butylphenol (2, 4-DTBP), a compound derived from Houttuynia cordata Thunb. To overcome the poor solubility of 2, 4-DTBP, we encapsulated it in polymeric micelles and delivered it by inhalation. We found that 2, 4-DTBP-loaded micelles inhibited RSV infection in vitro and improved survival, lung pathology, and viral clearance in RSV-infected mice. Our results suggested that 2, 4-DTBP-loaded micelle is a promising novel therapeutic agent for RSV infection.

Keywords: 2, 4-di-tert-butylphenol; Anti-virus; Human respiratory syncytial virus; In vitro; In vivo; Micelle.