Persistence of vaccine origin Salmonella Typhimurium through the poultry production continuum, and development of a rapid typing scheme for their differentiation from wild type field isolates

Timothy J Johnson; Cristian Flores-Figueroa; Jeannette Munoz-Aguayo; Glenda Pinho; Elizabeth Miller

doi:10.1016/j.psj.2024.103707

Persistence of vaccine origin Salmonella Typhimurium through the poultry production continuum, and development of a rapid typing scheme for their differentiation from wild type field isolates

Poult Sci. 2024 Mar 30;103(6):103707. doi: 10.1016/j.psj.2024.103707. Online ahead of print.

Authors

Timothy J Johnson¹, Cristian Flores-Figueroa², Jeannette Munoz-Aguayo², Glenda Pinho³, Elizabeth Miller³

Affiliations

¹ University of Minnesota, Department of Veterinary and Biomedical Sciences, Saint Paul, MN 55108, USA; University of Minnesota, Mid-Central Research and Outreach Center, Willmar, MN 56201, USA. Electronic address: tjj@umn.edu.
² University of Minnesota, Department of Veterinary and Biomedical Sciences, Saint Paul, MN 55108, USA; University of Minnesota, Mid-Central Research and Outreach Center, Willmar, MN 56201, USA.
³ University of Minnesota, Department of Veterinary and Biomedical Sciences, Saint Paul, MN 55108, USA.

Abstract

Salmonella enterica serovar Typhimurium is one of the top Salmonella serovars annually linked to poultry production and corresponding human illnesses. Because of this, vaccination of commercial poultry against Salmonella Typhimurium has been a focal point in recent years. There are several commercially available Salmonella Typhimurium vaccines available for use in poultry production. Among these are modified live vaccines, including Poulvac ST (Zoetis), Megan Egg (AviPro), and Megan Vac 1 (AviPro). In this study, analyses of 27 field isolates of Salmonella Typhimurium from poultry sources indicated evidence for the persistence of some vaccine-origin strains through the commercial production cycle. Further analyses of 26,812 database isolates indicated vaccine-origin isolates are persisting frequently through processing, are present on retail meat products, and are even occasionally found in human patients. A novel polymerase chain reaction (PCR) was created and validated which enables simultaneous identification of Salmonella enterica sp., the Salmonella Typhimurium serovar, and differentiation of wild type Salmonella Typhimurium from live attenuated vaccines involving mutations in the cya/crp or aroA genes. The PCR was developed considering whole genome differences between the vaccines and wild type field isolates and was validated using different field isolates and recovered vaccine strains. This method enables poultry producers to rapidly determine if recovered field isolates have a vaccine origin.

Keywords: Salmonella; Typhimurium; persistence; poultry; vaccine.