Objective: To investigate the microbiota composition and diversity between autogenous and anautogenous Culex pipiens pallens, so as to provide insights into unraveling the pathogenesis of autogeny in Cx. pipiens pallens.
Methods: Autogenous and anautogenous adult Cx. pipiens pallens samples were collected at 25 ℃, and the hypervariable regions of the microbial 16S ribosomal RNA (16S rRNA) gene was sequenced on the Illumina NovaSeq 6000 sequencing platform. The microbiota abundance and diversity were evaluated using the alpha diversity index, and the difference in the microbiota structure was examined using the beta diversity index. The microbiota with significant differences in the abundance between autogenous and anautogenous adult Cx. pipiens pallens samples was identified using the linear discriminant analysis effect size (LEfSe).
Results: The microbiota in autogenous and anautogenous Cx. pipiens pallens samples belonged to 18 phyla, 28 classes, 70 orders, 113 families, and 170 genera, and the dominant phyla included Proteobacteria, Bacteroidetes, and so on. At the genus level, Wolbachia was a common dominant genus, and the relative abundance was (77.6 ± 11.3)% in autogenous Cx. pipiens pallens samples and (47.5 ± 8.5)% in anautogenous mosquito samples, while Faecalibaculum (0.4% ± 0.1%), Dubosiella (0.5% ± 0.0%) and Massilia (0.5% ± 0.1%) were specific species in autogenous Cx. pipiens pallens samples. Alpha diversity analysis showed that higher Chao1 index and ACE index in autogenous Cx. pipiens pallens samples than in anautogenous samples (both P values > 0.05), and lower Shannon index (P > 0.05) and Simpson index (P < 0.05) in autogenous Cx. pipiens pallens samples than in anautogenous samples. LEfSe analysis showed a total of 48 significantly different taxa between autogenous and anautogenous Cx. pipiens pallens samples (all P values < 0.05).
Conclusions: There is a significant difference in the microbiota diversity between autogenous and anautogenous Cx. pipiens pallens.
［摘要］ 目的 了解自育与非自育淡色库蚊体内菌群组成与多样性差异, 从而为探索淡色库蚊自育发生机制提供参考 依据。方法 收集25 ℃自育与非自育淡色库蚊成蚊, 利用Illumina NovaSeq 6000测序平台对细菌16S核糖体RNA基因 高变区进行测序。采用α多样性指数分析菌群丰富度和多样性, 采用β多样性指数分析菌群结构差异, 通过线性判别分 析效应大小 (linear discriminant analysis effect size, LEfSe) 鉴别组间在丰度上具有显著差异的菌群。结果 自育与非自育 淡色库蚊体内菌群隶属18个门、28个纲、70个目、113个科和170个属, 优势菌门主要有变形菌门、拟杆菌门等。在属水 平, 沃尔巴克氏体菌属为共有优势菌属, 在自育及非自育淡色库蚊中的相对丰度分别为 (77.6 ± 11.3) %和 (47.5 ± 8.5) %; 粪杆菌属 (0.4% ± 0.1%)、杜氏杆菌属 (0.5% ± 0.0%)、马赛菌属 (0.5% ± 0.1%) 等为自育淡色库蚊特有菌属。α多样性分 析显示, 自育淡色库蚊体内菌群Chao1指数和ACE指数大于非自育淡色库蚊 (P 均> 0.05), 而Shannon指数 (P > 0.05) 和 Simpson指数 (P < 0.05) 小于非自育淡色库蚊。LEfSe分析结果显示, 共计48个分类单元在自育和非自育淡色库蚊间存 在显著差异 (P均< 0.05)。结论 自育与非自育淡色库蚊体内菌群多样性存在显著差异。.
Keywords:
16S ribosomal RNA; Alpha diversity; Autogeny; Beta diversity;