Microenvironment-regulated dual-hydrophilic coatings for glaucoma valve surface engineering

Shimeng Zhang; Yejia Liu; Linhua Li; Binjian Wang; Zezhen Zhang; Shiyan Chen; Guanghong Zhang; Qiongjian Huang; Xiao Chen; Jiang Chen; Chao Qu

doi:10.1016/j.actbio.2024.04.003

Microenvironment-regulated dual-hydrophilic coatings for glaucoma valve surface engineering

Acta Biomater. 2024 Apr 9:S1742-7061(24)00176-4. doi: 10.1016/j.actbio.2024.04.003. Online ahead of print.

Authors

Shimeng Zhang¹, Yejia Liu², Linhua Li³, Binjian Wang², Zezhen Zhang², Shiyan Chen², Guanghong Zhang², Qiongjian Huang⁴, Xiao Chen⁵, Jiang Chen⁶, Chao Qu⁷

Affiliations

¹ The Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Sichuan Provincial Key Laboratory for Human Disease Gene Study, the Department of Medical Genetics, the Institute of Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu, China.
² The Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
³ Kidney Research Institute, Division of Nephrology, West China Hospital of Sichuan University, Chengdu, 610041, China.
⁴ Chongqing Industry Polytechnic College, Chongqing 401120, China.
⁵ Institute of Biomaterials and Surface Engineering, Key Laboratory for Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, People's Republic of China. Electronic address: 382051858@qq.com.
⁶ The Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Sichuan Provincial Key Laboratory for Human Disease Gene Study, the Department of Medical Genetics, the Institute of Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu, China. Electronic address: 283876533@qq.com.
⁷ The Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Sichuan Provincial Key Laboratory for Human Disease Gene Study, the Department of Medical Genetics, the Institute of Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu, China. Electronic address: lucyjeffersonqu@hotmail.com.

PMID: 38604464
DOI: 10.1016/j.actbio.2024.04.003

Abstract

Glaucoma valves (GVs) play an essential role in treating glaucoma. However, fibrosis after implantation has limited their long-term success in clinical applications. In this study, we aimed to develop a comprehensive surface-engineering strategy to improve the biocompatibility of GVs by constructing a microenvironment-regulated and dual-hydrophilic antifouling coating on a GV material (silicone rubber, SR). The coating was based on a superhydrophilic polydopamine (SPD) coating with good short-range superhydrophilicity and antifouling abilities. In addition, SPD coatings contain many phenolic hydroxyl groups that can effectively resist oxidative stress and the inflammatory microenvironment. Furthermore, based on its in situ photocatalytic free-radical polymerization properties, the SPD coating polymerized poly 2-methylacryloxyethylphosphocholine, providing an additional long-range hydrophilic and antifouling effect. The in vitro test results showed that the microenvironment-regulated and dual-hydrophilic coatings had anti-protein contamination, anti-oxidation, anti-inflammation, and anti-fiber proliferation capabilities. The in vivo test results indicated that this coating substantially reduced the fiber encapsulation formation of the SR material by inhibiting inflammation and fibrosis. This design strategy for dual hydrophilic coatings with microenvironmental regulation can provide a valuable reference for the surface engineering design of novel medical implantable devices. STATEMENT OF SIGNIFICANCE: Superhydrophilic polydopamine (SPD) coatings were prepared on silicone rubber (SR) by a two-electron oxidation method. Introduction of pMPC to SPD surface using photocatalytic radical polymerization to obtain a dual-hydrophilic coating. The dual-hydrophilic coating effectively modulates the oxidative and inflammatory microenvironment. This coating significantly reduced protein contamination and adhesion of inflammatory cells and fibroblasts in vitro. The coating-modified SR inhibits inflammatory and fibrosis responses in vivo, promising to serve the glaucoma valves.

Keywords: Fibrosis; Glaucoma; Glaucoma valves; Inflammation; Super-hydrophilic polydopamine coating.