Lacticaseibacillusparacasei BNCC345679 revolutionizes DSS-induced colitis and modulates gut microbiota

Waqar Ahmad; Ahmad Ud Din; Taj Malook Khan; Mujeeb Ur Rehman; Adil Hassan; Tariq Aziz; Metab Alharbi; Jianbo Wu

doi:10.3389/fmicb.2024.1343891

Lacticaseibacillusparacasei BNCC345679 revolutionizes DSS-induced colitis and modulates gut microbiota

Front Microbiol. 2024 Mar 27:15:1343891. doi: 10.3389/fmicb.2024.1343891. eCollection 2024.

Authors

Waqar Ahmad^#¹, Ahmad Ud Din^#^{1

2}, Taj Malook Khan¹, Mujeeb Ur Rehman³, Adil Hassan⁴, Tariq Aziz⁵, Metab Alharbi⁶, Jianbo Wu^{1

7

8}

Affiliations

¹ Basic Medicine Research Innovation Centre for Cardiometabolic Diseases, Ministry of Education, Southwest Medical University, Luzhou, China.
² Plants for Human Health Institute, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Kannapolis, NC, United States.
³ Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
⁴ Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing, China.
⁵ Department of Agriculture, University of Ioannina, Ioannina, Greece.
⁶ Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
⁷ Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research of Southwest Medical University, Luzhou, China.
⁸ Laboratory for Cardiovascular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, China.

^# Contributed equally.

Abstract

The gut microbiota plays an important role in the disease progression of inflammatory bowel disease. Although probiotics are effective against IBD, not many studies have investigated their effects on gut microbiota composition and immunomodulation in mouse colitis models. Our study aimed at the therapeutic effects of Lacticaseibacillus paracasei BNCC345679 for the first time and explored its impact on gut microbiome dysbiosis, inflammatory cytokines, related miRNAs, VCAM-1, oxidative stress, intestinal integrity, and mucus barrier. We found that oral intervention of L. paracasei BNCC345679 affects recovering beneficial microbial taxa, including lactobacillus spp. and akkermansia spp., followed by improved body weight, DAI score, and inflammatory cytokines. L. paracasei BNCC345679 mitigated oxidative stress and increased the expression of intestinal integrity proteins MUC2 and ZO-1. These results suggested that L. paracasei BNCC345679 has the capacity to reduce DSS-induced colitis and has the potential as a supplement for the mitigation of IBD.

Keywords: DSS-induced colitis; Lacticaseibacillus paracasei BNCC345679; dysbiosis; gut inflammation; gut microbiota; microRNA; probiotics.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was supported by Joint Innovation Special Project of Science and Technology Plan of Sichuan Province (2022YFS0617) to JW, Southwest Medical University postdoctoral grant (00040177) to WA, and Luzhou-Southwest Medical University Cooperation Project (23YKDCXY0003).