An immune sandwich electrochemical biosensor based on triple-modified zirconium derivatives for detection of CD146 in serum

Die Chen; Yutao He; Ya Wang; Ziyi Zhang; Yifei Pei; Yumeng Lei; Junrui Hu; Shiqiang Xiang; Nicole Jaffrezic-Renault; Zhenzhong Guo

doi:10.1016/j.colsurfb.2024.113902

An immune sandwich electrochemical biosensor based on triple-modified zirconium derivatives for detection of CD146 in serum

Colloids Surf B Biointerfaces. 2024 Apr 6:239:113902. doi: 10.1016/j.colsurfb.2024.113902. Online ahead of print.

Authors

Die Chen¹, Yutao He¹, Ya Wang¹, Ziyi Zhang¹, Yifei Pei¹, Yumeng Lei², Junrui Hu³, Shiqiang Xiang⁴, Nicole Jaffrezic-Renault⁵, Zhenzhong Guo⁶

Affiliations

¹ Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Medicine, School of Public Health, Faculty of Medicine, Wuhan University of Science and Technology, Wuhan 430065, PR China.
² Department of Medical Ultrasound, China Resources & Wisco General Hospital, Wuhan University of Science and Technology, Wuhan 430080, PR China.
³ Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.
⁴ Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Medicine, School of Public Health, Faculty of Medicine, Wuhan University of Science and Technology, Wuhan 430065, PR China. Electronic address: xiangshiqiang@wust.edu.cn.
⁵ University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, Villeurbanne 69100, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.
⁶ Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Medicine, School of Public Health, Faculty of Medicine, Wuhan University of Science and Technology, Wuhan 430065, PR China. Electronic address: zhongbujueqi@hotmail.com.

PMID: 38599037
DOI: 10.1016/j.colsurfb.2024.113902

Abstract

CD146, also known as melanoma cell adhesion molecule (MCAM), is overexpressed in various cancer patients, making it a valuable predictor for early diagnosis. In this work, an immune sandwich electrochemical biosensor is proposed for sensitive and non-invasive quantitative detection of CD146 in serum. Zirconium-based MOF (UIO-66) was modified by simultaneous copper atom doping, in situ growth carbon-based support and physical embedding of platinum nanoparticles (PtNPs). Triple-modified Cu-UIO-66@SWCNT/PtNPs nanocomposites with high stability and excellent electrochemical properties, serve as surface modification materials for glassy carbon electrodes. Anti-CD146 antibody (Ab₁) was grafted onto the electrode surface via Pt-S bond. Meanwhile, the secondary antibody (Ab₂) was conjugated with silver nanoparticles (AgNPs) to cooperate for CD146 capture and achieve secondary electrical signal amplification. Under optimal conditions, square wave voltammetry was employed to determine CD146 in the concentration range of 10^-9-10^-4 mg/mL and a limit of detection of 12 fg/mL was obtained. Finally, it was successfully applied to the analysis of CD146 in lung and liver cancer patients' serum samples.

Keywords: CD146; Hepatocellular carcinoma (HCC); Non-small cell lung cancer (NSCLC); Sandwich pattern biosensor; Triple-modified zirconium-based MOFs.