The Impact of FBN1-α5β1 Axis in Fibro/Adipogenic Progenitor Cells (FAPCD9-) on Intramuscular Fat Content in Pigs

Xian Tong; Qi Zhu; Tianqi Duo; Ziyun Liang; Chong Zhang; Shufang Cai; Xiaoyu Wang; Yihao Liu; Yongpeng Li; Xiaohong Liu; Zuyong He; Bin Hu; Jianhua Zeng; Yaosheng Chen; Delin Mo

doi:10.1021/acs.jafc.4c00059

The Impact of FBN1-α5β1 Axis in Fibro/Adipogenic Progenitor Cells (FAP^CD9-) on Intramuscular Fat Content in Pigs

J Agric Food Chem. 2024 Apr 10. doi: 10.1021/acs.jafc.4c00059. Online ahead of print.

Authors

Xian Tong¹, Qi Zhu¹, Tianqi Duo¹, Ziyun Liang¹, Chong Zhang¹, Shufang Cai¹, Xiaoyu Wang¹, Yihao Liu¹, Yongpeng Li¹, Xiaohong Liu¹, Zuyong He¹, Bin Hu², Jianhua Zeng³, Yaosheng Chen¹, Delin Mo¹

Affiliations

¹ State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong 510275, China.
² Guangdong Key Laboratory of Animal Breeding and Nutrition, State Key, Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China.
³ Guangdong YIHAO Food Co.,Ltd., Guangzhou 510620, China.

PMID: 38598771
DOI: 10.1021/acs.jafc.4c00059

Abstract

Intramuscular fat (IMF) plays a crucial role in enhancing meat quality, enriching meat flavor, and overall improving palatability. In this study, Single-cell RNA sequencing was employed to analyze the longissimus dorsi (LD) obtained from Guangdong small-ear spotted pigs (GDSS, with high IMF) and Yorkshire pigs (YK, with low IMF). GDSS had significantly more Fibro/Adipogenic Progenitor (FAPs), in which the CD9 negative FAPs (FAP^CD9-) having adipogenic potential, as demonstrated by in vitro assays using cells originated from mouse muscle. On the other hand, Yorkshire had more fibro-inflammatory progenitors (FIPs, marked with FAP^CD9+), presenting higher expression of the FBN1-Integrin α5β1. FBN1-Integrin α5β1 could inhibit insulin signaling in FAP^CD9-, suppressing adipogenic differentiation. Our results demonstrated that fat-type pigs possess a greater number of FAP^CD9-, which are the exclusive cells in muscle capable of differentiating into adipocytes. Moreover, lean-type pigs exhibit higher expression of FBN1-Integrin α5β1 axis, which inhibits adipocyte differentiation. These results appropriately explain the observed higher IMF content in fat-type pigs.

Keywords: FBN1-Integrin α5β1 axis; Fibro/Adipogenic Progenitor Cells; intramuscular fat; pig; scRNA-seq.