A cubic DNA nanocage probe for in situ analysis of miRNA-10b in tumor-derived extracellular vesicles

Xiaoyan Sun; Yafei Chen; Haiyan Li; Wei Xing; Mingli Chen; Jianhua Wang; Lei Ye

doi:10.1039/d4cc01049c

A cubic DNA nanocage probe for in situ analysis of miRNA-10b in tumor-derived extracellular vesicles

Chem Commun (Camb). 2024 Apr 30;60(36):4777-4780. doi: 10.1039/d4cc01049c.

Authors

Xiaoyan Sun¹, Yafei Chen¹, Haiyan Li¹, Wei Xing¹, Mingli Chen^{1

2}, Jianhua Wang¹, Lei Ye²

Affiliations

¹ Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang, Liaoning, 110819, P. R. China. chenml@mail.neu.edu.cn.
² Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box124, 22100 Lund, Sweden. lei.ye@tbiokem.lth.se.

PMID: 38597795
DOI: 10.1039/d4cc01049c

Abstract

A cubic DNA nanocage probe is able to enter EVs derived from MDA-MB-231 cells and react with miRNA-10b. The probe-loaded EVs were employed to monitor the process of entry of miRNA-10b into MCF-10A cells, allowing visualization of EV-mediated intercellular communication of miRNA-10b between the cancer cells.

MeSH terms

Cell Line, Tumor
DNA Probes / chemistry
Extracellular Vesicles* / chemistry
Extracellular Vesicles* / metabolism
Humans
MicroRNAs* / analysis
MicroRNAs* / metabolism
Nanostructures / chemistry

Substances

MicroRNAs
MIRN10 microRNA, human
DNA Probes