Proteomic analysis of the serum in dogs with pulmonary hypertension secondary to myxomatous mitral valve disease: the preliminary study

Siriwan Sakarin; Anudep Rungsipipat; Sittiruk Roytrakul; Janthima Jaresitthikunchai; Narumon Phaonakrop; Sawanya Charoenlappanit; Siriwan Thaisakun; Sirilak Disatian Surachetpong

doi:10.3389/fvets.2024.1327453

Proteomic analysis of the serum in dogs with pulmonary hypertension secondary to myxomatous mitral valve disease: the preliminary study

Front Vet Sci. 2024 Mar 26:11:1327453. doi: 10.3389/fvets.2024.1327453. eCollection 2024.

Authors

Siriwan Sakarin¹, Anudep Rungsipipat², Sittiruk Roytrakul³, Janthima Jaresitthikunchai³, Narumon Phaonakrop³, Sawanya Charoenlappanit³, Siriwan Thaisakun³, Sirilak Disatian Surachetpong¹

Affiliations

¹ Faculty of Veterinary Science, Department of Veterinary Medicine, Center of Excellence for Companion Animal Cancer, Chulalongkorn University, Bangkok, Thailand.
² Faculty of Veterinary Science, Department of Pathology, Chulalongkorn University, Bangkok, Thailand.
³ Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand.

Abstract

Background: Pulmonary hypertension (PH) is a common complication in dogs with myxomatous mitral valve disease (MMVD), characterized by elevated blood pressure in pulmonary artery. Echocardiography is a reliable technique for PH diagnosis in veterinary medicine. However, it is limited to use as an early detection method. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has found extensive application in the discovery of serum protein biomarkers for various diseases. The objective of this study was to identify serum proteins in healthy control dogs and MMVD dogs both with and without PH using LC-MS/MS.

Materials and methods: In this research, a total of 81 small-breed dogs participated, and they were categorized into three groups: the control (n = 28), MMVD (n = 24) and MMVD+PH (n = 29) groups. Serum samples were collected and analyzed by LC-MS/MS.

Results: Differentially expressed proteins were identified, and the upregulated and downregulated proteins in MMVD+PH group including Myomesin 1 (MYOM1) and Histone deacetylase 7 (HDAC7), Pleckstrin homology domain containing M3 (PLEKHM3), Diacylglycerol lipase alpha (DAGLA) and Tubulin tyrosine ligase like 6 (TTLL6) were selected as proteins of interest in MMVD dogs with PH.

Conclusion: Different types of proteins have been identified in healthy dogs and MMVD dogs with and without PH. Additional studies are needed to investigate the potential of these proteins as biomarkers for PH in dogs with MMVD.

Keywords: biomarkers; dogs; myxomatous mitral valve disease; proteomics; pulmonary hypertension; serum.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The study was funded by the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund) and the 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarship.