Molecular Interactions Between Egg White Peptides and Giant Unilamellar Vesicle Membranes: Effect of Peptide Localization on Membrane Fluidity

Ting Zhang; Menghan Fu; Ting Yu; Feng Jiang; Siwen Lyu; Qi Yang; Zhiyang Du; Xuanting Liu; Jingbo Liu; Yiding Yu

doi:10.1021/acs.jafc.3c08291

Molecular Interactions Between Egg White Peptides and Giant Unilamellar Vesicle Membranes: Effect of Peptide Localization on Membrane Fluidity

J Agric Food Chem. 2024 Apr 9. doi: 10.1021/acs.jafc.3c08291. Online ahead of print.

Authors

Ting Zhang^{1

2}, Menghan Fu^{1

2}, Ting Yu³, Feng Jiang^{1

2

4}, Siwen Lyu^{1

2}, Qi Yang^{1

2}, Zhiyang Du^{1

2}, Xuanting Liu^{1

2}, Jingbo Liu^{1

2}, Yiding Yu^{1

2}

Affiliations

¹ Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, People's Republic of China.
² College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China.
³ Department of Nutrition, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China.
⁴ Department of Molecular & Integrative Physiology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, United States.

PMID: 38592417
DOI: 10.1021/acs.jafc.3c08291

Abstract

Bioactive peptides have been shown to affect cell membrane fluidity, which is an important indicator of the cell membrane structure and function. However, the underlying mechanism of egg white-derived bioactive peptide regulation of cell membrane fluidity has not been elucidated yet. The cell membrane fluidity was investigated by giant unilamellar vesicles in the present study. The results showed that peptides TCNW, ADWAK, ESIINF, VPIEGII, LVEEY, and WKLC connect to membranes through intermolecular interactions, such as hydrogen bonding and regulated membrane fluidity, in a concentration-dependent way. In addition, peptides prefer to localize in the hydrophobic core of the bilayers. This study provides a theoretical basis for analyzing the localization of egg white bioactive peptides in specific cell membrane regions and their influence on the cell membrane fluidity.

Keywords: egg white peptide; giant unilamellar vesicles; membrane fluidity; peptide localization.