Pressurized liquid extraction of glucosinolates from Camelina sativa (L.) Crantz by-products: Process optimization and biological activities of green extract

Stefania Pagliari; Gloria Domínguez-Rodríguez; Alejandro Cifuentes; Elena Ibáñez; Massimo Labra; Luca Campone

doi:10.1016/j.fochx.2024.101324

Pressurized liquid extraction of glucosinolates from Camelina sativa (L.) Crantz by-products: Process optimization and biological activities of green extract

Food Chem X. 2024 Mar 24:22:101324. doi: 10.1016/j.fochx.2024.101324. eCollection 2024 Jun 30.

Authors

Stefania Pagliari¹, Gloria Domínguez-Rodríguez², Alejandro Cifuentes², Elena Ibáñez², Massimo Labra^{1

3}, Luca Campone^{1

3}

Affiliations

¹ Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy.
² Foodomics Laboratory, Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Nicolás Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.
³ NBFC, National Biodiversity Future Center, 90133 Palermo, Italy.

Abstract

The cultivation of Camelina sativa (L.) Crantz is rapidly increasing due to oil production resulting in a substantial volume of by-products, which still have an interesting composition in secondary metabolites, especially glucosinolates. Therefore, a green extraction procedure of glucosinolates by Pressurised Liquid Extraction was developed and optimized using a chemometric approach. Furthermore, the glucosinolates were purified by solid phase extraction, and a preliminary study on bioaccessibility and bioavailability study was carried out to evaluate the resistance of the glucosinolates to the digestive process. The application of pressurised liquid extraction to the recovery of glucosinolates from camelina sativa by-product, is a green, automatic, and rapid method, representing a valid alternative to conventional extraction method to obtain ingredients for food industries.

Keywords: Bioaccessibility; Camelina sativa by-products; Glucoarabin (PubChem CID 46173881); Glucocamelin (PubChem CID 162639109); Glucohirsutin (PubChem CID 44237258); Glucosinolates; Pressurized liquid extraction.