Single-virus-sensitive barcode qPCR mediated by the aggregation of gold nanoparticle probes

Yuanzhao Shen; Chih-Tsung Yang; Weiwei Li; Xin Zhou

doi:10.1039/d4an00121d

Single-virus-sensitive barcode qPCR mediated by the aggregation of gold nanoparticle probes

Analyst. 2024 Apr 29;149(9):2556-2560. doi: 10.1039/d4an00121d.

Authors

Yuanzhao Shen¹, Chih-Tsung Yang², Weiwei Li³, Xin Zhou¹

Affiliations

¹ College of Veterinary Medicine, Institute of Comparative Medicine, Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. zhou_xin@126.com.
² Future Industries Institute, University of South Australia, Mawson Lakes Blvd, Mawson Lakes, South Australia 5095, Australia.
³ Institute of Pediatrics, Children's Hospital of Fudan University, Fudan University, Shanghai 201102, China. weiweili@fudan.edu.cn.

PMID: 38587837
DOI: 10.1039/d4an00121d

Abstract

Herein, we developed a gold nanoparticle (GNP)-mediated barcode qPCR strategy with a sensitivity for a single virus particle per reaction for the detection of influenza virus H3N2. The analysis of the results for pure virus and real virus samples show that GNP-mediated barcode qPCR is ∼16 times more sensitive than conventional qPCR, demonstrating the potential to reduce false negatives and improve early diagnosis of viral infections.

MeSH terms

Gold* / chemistry
Humans
Influenza A Virus, H3N2 Subtype* / genetics
Influenza A Virus, H3N2 Subtype* / isolation & purification
Metal Nanoparticles* / chemistry
Real-Time Polymerase Chain Reaction* / methods

Substances

Gold