Glucose Oxidase Activity Colorimetric Assay Using Redox-Sensitive Electrochromic Nanoparticle-Functionalized Paper Sensors

Taeha Lee; Jeongmin Park; Seung Hyeon Oh; Da Yeon Cheong; Seokbeom Roh; Jae Hyun You; Yoochan Hong; Gyudo Lee

doi:10.1021/acsomega.4c00335

Glucose Oxidase Activity Colorimetric Assay Using Redox-Sensitive Electrochromic Nanoparticle-Functionalized Paper Sensors

ACS Omega. 2024 Mar 20;9(13):15493-15501. doi: 10.1021/acsomega.4c00335. eCollection 2024 Apr 2.

Authors

Taeha Lee^{1

2}, Jeongmin Park¹, Seung Hyeon Oh^{1

2}, Da Yeon Cheong^{1

2}, Seokbeom Roh^{1

2}, Jae Hyun You³, Yoochan Hong⁴, Gyudo Lee^{1

2}

Affiliations

¹ Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, South Korea.
² Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong 30019, South Korea.
³ Division of Convergence Business, Korea University, Sejong 30019, South Korea.
⁴ Department of Medical Device, Korea Institute of Machinery and Materials (KIMM), Daegu 42994, South Korea.

Abstract

Glucose oxidase (GOx) activity assays are vital for various applications, including glucose metabolism estimation and fungal testing. However, conventional methods involve time-consuming and complex procedures. In this study, we present a colorimetric platform for in situ GOx activity measurement utilizing redox-sensitive electrochromic nanoparticles based on polyaniline (PAni). The glucose-adsorbed colorimetric paper sensor, herein termed Glu@CPS, is created by immobilizing ferrocene and glucose onto paper substrates that have been functionalized with PAni nanoparticles. Glu@CPS not only demonstrated rapid detection (within 5 min) but also exhibited remarkable selectivity for GOx and a limit of detection as low as 1.25 μM. Moreover, Glu@CPS demonstrated consistent accuracy in the measurement of GOx activity, exhibiting no deviations even after being stored at ambient temperature for a duration of one month. To further corroborate the effectiveness of this method, we applied Glu@CPS in the detection of GOx activity in a moldy red wine. The results highlight the promising potential of Glu@CPS as a convenient and precise platform for GOx activity measurement in diverse applications including food quality control, environmental monitoring, and early detection of fungal contamination.