Cytokines and chemokines involved in HLA-B27-positive ankylosing spondylitis-associated acute anterior uveitis

Huan Li; Zhaoxia He; Bolin Deng; Chen Yang; Liang Wang; Jialing Xiao; Weijia Wu; Xiangmei Li; Lixin Zhang; Yutong Wei; Siyu Zhu; Huining Yang; Huanyue Hai; Jiarui Hu; Lin Li; Yi Shi; Man Yu; Ping Shuai; Yuping Liu; Xueming Ju; Gang Wu; Yu Zhou; Jing Zhu; Bo Gong

Cytokines and chemokines involved in HLA-B27-positive ankylosing spondylitis-associated acute anterior uveitis

Mol Vis. 2023 Dec 30:29:378-385. eCollection 2023.

Authors

Huan Li^{1

2

3}, Zhaoxia He⁴, Bolin Deng⁵, Chen Yang⁶, Liang Wang^{1

2

3}, Jialing Xiao^{1

2

3}, Weijia Wu^{1

2

3}, Xiangmei Li^{1

2

3}, Lixin Zhang^{1

2

3}, Yutong Wei^{1

2

3}, Siyu Zhu^{1

2

3}, Huining Yang^{1

2

3}, Huanyue Hai^{1

2

3}, Jiarui Hu^{1

2

3}, Lin Li², Yi Shi², Man Yu⁵, Ping Shuai¹, Yuping Liu¹, Xueming Ju¹, Gang Wu¹, Yu Zhou^{1

2

3}, Jing Zhu⁶, Bo Gong^{1

2

3}

Affiliations

¹ Department of Health Management, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
² Human Disease Genes Key Laboratory of Sichuan Province and Institute of Laboratory Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
³ Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
⁴ Department of Health Management, Sichuan Academy of Medical Sciences & The People's Hospital of Wenjiang, Chengdu, Sichuan, China.
⁵ Department of Ophthalmology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
⁶ Department of Rheumatology and Immunology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

PMID: 38577559
PMCID: PMC10994677

Abstract

Purpose: Acute anterior uveitis (AAU) is the most common extra-articular symptom of ankylosing spondylitis (AS). This study aims to reveal the cytokines and chemokines involved in the immunopathogenesis of human leucocyte antigen (HLA)-B27⁺ AS-associated AAU.

Methods: Twenty-one HLA-B27⁺ AS-associated AAU patients and 21 healthy controls (HCs) were recruited for this study. Serum cytokine concentrations in all 42 subjects were determined by the Meso Scale Discovery (MSD) electrochemiluminescence method. In each sample, 34 cytokines, 10 chemokines, eight angiogenesis mediators, and four vascular injury mediators were measured. The differences in cytokine and chemokine concentrations were compared between the two groups.

Results: Concentrations of serum IL-3, TNF-α, IL-6, IL-17D, IL-22, IP10/CXCL10, MIP-3α/CCL20, sFlt-1/VEGFR-1, CRP, and MCP-4/CCL13 were significantly higher in patients with HL-B27⁺ AS-associated AAU than in HCs (p < 0.05). In contrast, concentrations of serum IL-4, IL-8, MIP-1α/CCL3, Eotaxin-3/CCL26, PlGF, VEGF-C, and VEGF-D were significantly lower in patients with HL-B27⁺ AS-associated AAU than in HCs (p < 0.05).

Conclusions: Significant differences were detected in the levels of several cytokines and chemokines in the serum of HLA-B27⁺ AS-associated AAU compared with HCs. Some novel differential cytokines and chemokines that have not been reported in other kinds of uveitis were also identified. These results reveal the underlying pathogenesis of HLA-B27⁺ AS-associated AAU and could potentially aid in clinical diagnosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Disease
Chemokines
Cytokines
HLA-B27 Antigen / genetics
Humans
Spondylitis, Ankylosing* / complications
Uveitis, Anterior*

Substances

Cytokines
HLA-B27 Antigen
Chemokines