A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus

Hua Cao; Mengjia Zhang; Zheyu Liao; Dongfan Li; Xinglin He; Hailong Ma; Pengfei Li; Xuexiang Yu; Guiqing Peng; Shengsong Xie; Qigai He; Wentao Li

doi:10.1186/s13567-024-01300-2

A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus

Vet Res. 2024 Apr 4;55(1):42. doi: 10.1186/s13567-024-01300-2.

Authors

Hua Cao^#^{1

2

3}, Mengjia Zhang^#^{1

2

3}, Zheyu Liao^{1

2

3}, Dongfan Li^{1

2

3}, Xinglin He^{1

2

3}, Hailong Ma^{1

2

3}, Pengfei Li^{1

2

3}, Xuexiang Yu^{1

2

3}, Guiqing Peng^{1

2

3}, Shengsong Xie⁴, Qigai He^{5

6}, Wentao Li^{7

8

9}

Affiliations

¹ National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
² Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, China.
³ Hubei Hongshan Laboratory, Wuhan, 430070, China.
⁴ Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
⁵ National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. he628@mail.hzau.edu.cn.
⁶ Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, China. he628@mail.hzau.edu.cn.
⁷ National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. wentao@mail.hzau.edu.cn.
⁸ Key Laboratory of Prevention and Control for African Swine Fever and Other Major Pig Diseases, Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, China. wentao@mail.hzau.edu.cn.
⁹ Hubei Hongshan Laboratory, Wuhan, 430070, China. wentao@mail.hzau.edu.cn.

^# Contributed equally.

Abstract

African Swine Fever virus (ASFV), the causative agent of African swine fever, is a highly lethal hemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs), which are difficult to obtain and maintain in vitro, and less subjective to genetic editing. To overcome these issues and facilitate ASFV research, we obtained a subclonal cell line PK1-C5 by subcloning LLC-PK1 cells that support stable ASFV proliferation. This consequential cell line exhibited high ASFV infection levels and similar viral growth characteristics to PAMs, while also allowing high-efficiency genomic editing through transfection or lentivirus transduction of Cas9. Taken together, our study provided a valuable tool for research aspects including ASFV-host interactions, pathogenicity, and vaccine development.

Keywords: African swine fever virus (ASFV); LLC-PK1; genome editing.

MeSH terms

African Swine Fever Virus* / genetics
African Swine Fever*
Animals
Cell Line
Kidney
Sus scrofa
Swine
Swine Diseases*

Abstract

MeSH terms

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