GSNOR negatively regulates the NLRP3 inflammasome via S-nitrosation of MAPK14

Qianjin Liu; Lijin Jiao; Mao-Sen Ye; Zhiyu Ma; Jinsong Yu; Ling-Yan Su; Wei-Yin Zou; Lu-Xiu Yang; Chang Chen; Yong-Gang Yao

doi:10.1038/s41423-024-01155-9

GSNOR negatively regulates the NLRP3 inflammasome via S-nitrosation of MAPK14

Cell Mol Immunol. 2024 Apr 3. doi: 10.1038/s41423-024-01155-9. Online ahead of print.

Authors

Qianjin Liu¹, Lijin Jiao², Mao-Sen Ye², Zhiyu Ma^{2

3}, Jinsong Yu², Ling-Yan Su², Wei-Yin Zou^{2

3}, Lu-Xiu Yang², Chang Chen⁴, Yong-Gang Yao^{5

6

7

8}

Affiliations

¹ Key Laboratory of Genetic Evolution & Animal Models, and Key Laboratory of Animal Models & Human Disease Mechanisms of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 650201, Kunming, Yunnan, China. liuqianjin@mail.kiz.ac.cn.
² Key Laboratory of Genetic Evolution & Animal Models, and Key Laboratory of Animal Models & Human Disease Mechanisms of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 650201, Kunming, Yunnan, China.
³ Kunming College of Life Science, University of Chinese Academy of Sciences, 650204, Kunming, Yunnan, China.
⁴ Key Laboratory of Biomacromolecules (CAS), National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China.
⁵ Key Laboratory of Genetic Evolution & Animal Models, and Key Laboratory of Animal Models & Human Disease Mechanisms of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 650201, Kunming, Yunnan, China. yaoyg@mail.kiz.ac.cn.
⁶ Kunming College of Life Science, University of Chinese Academy of Sciences, 650204, Kunming, Yunnan, China. yaoyg@mail.kiz.ac.cn.
⁷ KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, 650201, Kunming, Yunnan, China. yaoyg@mail.kiz.ac.cn.
⁸ National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), National Resource Center for Non-Human Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, 650201, Kunming, China. yaoyg@mail.kiz.ac.cn.

PMID: 38570588
DOI: 10.1038/s41423-024-01155-9

Abstract

Hyperactivation of the NLRP3 inflammasome has been implicated in the pathogenesis of numerous diseases. However, the precise molecular mechanisms that modulate the transcriptional regulation of NLRP3 remain largely unknown. In this study, we demonstrated that S-nitrosoglutathione reductase (GSNOR) deficiency in macrophages leads to significant increases in the Nlrp3 and Il-1β expression levels and interleukin-1β (IL-1β) secretion in response to NLRP3 inflammasome stimulation. Furthermore, in vivo experiments utilizing Gsnor^-/- mice revealed increased disease severity in both lipopolysaccharide (LPS)-induced septic shock and dextran sodium sulfate (DSS)-induced colitis models. Additionally, we showed that both LPS-induced septic shock and DSS-induced colitis were ameliorated in Gsnor^-/- Nlrp3^-/- double-knockout (DKO) mice. Mechanistically, GSNOR deficiency increases the S-nitrosation of mitogen-activated protein kinase 14 (MAPK14) at the Cys211 residue and augments MAPK14 kinase activity, thereby promoting Nlrp3 and Il-1β transcription and stimulating NLRP3 inflammasome activity. Our findings suggested that GSNOR is a regulator of the NLRP3 inflammasome and that reducing the level of S-nitrosylated MAPK14 may constitute an effective strategy for alleviating diseases associated with NLRP3-mediated inflammation.

Keywords: S-nitrosation; Colitis; GSNOR; MAPK14; NLRP3 inflammasome; Septic shock.