Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease

Riku Kinoshita; Naoya Inoue; Yoshinori Iwatani; Yusuke Noguchi; Yoh Hidaka; Mikio Watanabe

doi:10.1016/j.clim.2024.110196

Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease

Clin Immunol. 2024 Apr 1:263:110196. doi: 10.1016/j.clim.2024.110196. Online ahead of print.

Authors

Riku Kinoshita¹, Naoya Inoue², Yoshinori Iwatani¹, Yusuke Noguchi¹, Yoh Hidaka³, Mikio Watanabe⁴

Affiliations

¹ Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Yamadaoka 1-7 Suita, Osaka 565-0871, Japan.
² Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Yamadaoka 1-7 Suita, Osaka 565-0871, Japan; Laboratory for Clinical Investigation, Osaka University Hospital, Yamadaoka 2-15, Suita, Osaka 565-0871, Japan.
³ Laboratory for Clinical Investigation, Osaka University Hospital, Yamadaoka 2-15, Suita, Osaka 565-0871, Japan.
⁴ Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Yamadaoka 1-7 Suita, Osaka 565-0871, Japan. Electronic address: nabe@sahs.med.osaka-u.ac.jp.

PMID: 38570004
DOI: 10.1016/j.clim.2024.110196

Abstract

The prognosis of autoimmune thyroid diseases (AITDs), including Hashimoto's disease (HD) and Graves' disease (GD), is difficult to predict. DNA methylation regulates gene expression of immune mediating factors. Interleukin (IL)-10 is a Th2 cytokine that downregulates inflammatory cytokines produced by Th1 cells. To clarify the role of methylation of the IL10 gene in the prognosis of AITD, we evaluated the methylation levels of two CpG sites in the IL10 promoter using pyrosequencing. The methylation levels of the -185 CpG site of the IL10 gene were related to age and GD intractability in GD patients. Furthermore, the C carrier of the IL10-592 A/C polymorphism was related to low methylation levels of the -185 CpG site. The methylation levels of the IL10-185 CpG site of the IL10 gene were related to the intractability of GD and were lower in individuals with the C allele of the IL10-592 A/C polymorphism.

Keywords: Autoimmune thyroid disease; IL-10; Intractability; Methylation; Polymorphism; Pyrosequencing; Severity.