Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging

Tao Dai; Laurie J Rich; Mukund Seshadri; Subhamoy Dasgupta

doi:10.1016/j.xpro.2024.102993

Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging

STAR Protoc. 2024 Apr 1;5(2):102993. doi: 10.1016/j.xpro.2024.102993. Online ahead of print.

Authors

Tao Dai¹, Laurie J Rich², Mukund Seshadri³, Subhamoy Dasgupta⁴

Affiliations

¹ Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.
² FUJIFILM VisualSonics, Inc, Toronto, ON, Canada; Department of Oral Oncology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.
³ Department of Oral Oncology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA. Electronic address: mukund.seshadri@roswellpark.org.
⁴ Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA. Electronic address: subhamoy.dasgupta@roswellpark.org.

Abstract

Photoacoustic imaging (PAI) with co-registered ultrasound (US) is a hybrid non-invasive imaging modality that enables visualization and quantification of tumor hypoxia in live animals. Here, using a breast tumor xenograft model as an example, we present a stepwise protocol describing animal preparation, positioning, instrument setup, and US-PAI image acquisition procedures. This protocol also guides through detailed data analysis, explains functional readouts obtained from PAI, and discusses the potential application of the technology to study the hypoxic tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Dai et al.¹.

Keywords: Biophysics; Cancer; Cell Biology; Model Organisms.

Abstract

Grants and funding