Nanopore sensitization based on a double loop hybridization chain reaction and G-quadruplex

Yanru Li; Chunmiao Yu; Yesheng Wang; Jin Yu; Huaning Wang; Bingling Li

doi:10.1039/d4cc00125g

Nanopore sensitization based on a double loop hybridization chain reaction and G-quadruplex

Chem Commun (Camb). 2024 Apr 18;60(33):4487-4490. doi: 10.1039/d4cc00125g.

Authors

Yanru Li^{1

2}, Chunmiao Yu^{1

2}, Yesheng Wang^{1

2}, Jin Yu^{1

2}, Huaning Wang^{1

2}, Bingling Li^{1

2}

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. binglingli@ciac.ac.cn.
² School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, China.

PMID: 38567405
DOI: 10.1039/d4cc00125g

Abstract

The widespread implementation of solid-state nanopores faces challenges such as lower resolution and increased electrical noise when compared to biological nanopores. Incorporating specific nucleic acid reactions can enhance resolution. In this study, we've developed a nucleic acid amplifier to enhance the sensitivity of solid-state nanopores, utilizing a G-rich sequence and hybridization chain reaction. This amplifier improves target concentration and volume amplification, showing promise in nanopore sensitivity tests.