Bioguided isolation, identification and bioactivity evaluation of anti-fatigue constituents from Schizophyllum commune

Hao Chen; Qianzhen Wu; Kaili Xuan; Wenqiang Guo; Kunfeng Zhang; Yuanyuan Wang; Zhenzhen Dai; Rong Sheng; Huaqi He; Yuzhe Huang; Yan Chen

doi:10.1016/j.fitote.2024.105940

Bioguided isolation, identification and bioactivity evaluation of anti-fatigue constituents from Schizophyllum commune

Fitoterapia. 2024 Mar 31:175:105940. doi: 10.1016/j.fitote.2024.105940. Online ahead of print.

Authors

Hao Chen¹, Qianzhen Wu¹, Kaili Xuan¹, Wenqiang Guo¹, Kunfeng Zhang¹, Yuanyuan Wang², Zhenzhen Dai², Rong Sheng², Huaqi He³, Yuzhe Huang¹, Yan Chen⁴

Affiliations

¹ Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, School of Life Sciences, Anhui University, Hefei, Anhui, China.
² Anhui Zhongqing Testing Co., Ltd, Hefei, Anhui, China.
³ College of Agriculture, Anhui Science and Technology University, Chu Zhou, Anhui, China.
⁴ Key Laboratory of Ecological Engineering and Biotechnology of Anhui Province, School of Life Sciences, Anhui University, Hefei, Anhui, China. Electronic address: chenlab@ahu.edu.cn.

PMID: 38565382
DOI: 10.1016/j.fitote.2024.105940

Abstract

This study aims to clarify the specific anti-fatigue components of Schizophyllum commune (S.commune) and analyze its potential anti-fatigue mechanism. The main anti-fatigue active ingredient of S.commune was locked in n-butanol extract (SPE-n) by activity evaluation. Twelve compounds were identified by high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS). The anti-fatigue effect of morusin is the most predominant among these 12 ingredients. The determination of biochemical indices showed that morusin could increase liver glycogen reserves, improve the activity of antioxidant enzymes in liver, and reduce reactive oxygen species (ROS) content in muscle tissue, thereby reducing myocyte damage. Further studies revealed that morusin could reduce the level of oxidative stress by activating Nrf2/HO-1 pathway, thus alleviating the fatigue of mice caused by exhaustive exercise. The current findings provide a theoretical basis for the development of natural anti-fatigue functional food.

Keywords: Anti-fatigue; Morusin; Oxidative stress; Schizophyllum commune.