Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues

Li-Feng Yang; Ya-Ge Liu; Yun-Li Tao; Wan-Min Zhang; Jian-Yong Li; Sheng-Qi Chi; Gui-Fen Zhang; Dong Chu

doi:10.1002/ps.8114

Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues

Pest Manag Sci. 2024 Apr 2. doi: 10.1002/ps.8114. Online ahead of print.

Authors

Li-Feng Yang^{1

2}, Ya-Ge Liu^{1

2}, Yun-Li Tao^{1

2}, Wan-Min Zhang³, Jian-Yong Li⁴, Sheng-Qi Chi^{1

2}, Gui-Fen Zhang⁵, Dong Chu^{1

2}

Affiliations

¹ Shandong Engineering Research Center for Environment-friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
² Shandong Province Center for Bioinvasions and Eco-security, Qingdao Agricultural University, Qingdao, China.
³ Department of Pest Prediction and Management, Liaoning Technological Center of Green Agriculture, Shenyang, China.
⁴ Tech Center of Jinan Customs District, Jinan, China.
⁵ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Integrated Pest Management of Crop, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

PMID: 38563512
DOI: 10.1002/ps.8114

Abstract

Background: The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China.

Results: We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment.

Conclusion: In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China. © 2024 Society of Chemical Industry.

Keywords: Phthorimaea absoluta; invasive species; loop mediated isothermal amplification (LAMP); on‐site diagnostics.

Abstract

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