Facile Synthesis of PEGylated Gold Nanoparticles for Enhanced Colorimetric Detection of Histamine

Jahor Omping; Romnick Unabia; Renzo Luis Reazo; Melbagrace Lapening; Ryan Lumod; Archie Ruda; Rolen Brian Rivera; Noel Lito Sayson; Felmer Latayada; Rey Capangpangan; Gerard Dumancas; Roberto Malaluan; Arnold Lubguban; Gaudencio Petalcorin Jr; Arnold Alguno

doi:10.1021/acsomega.3c10050

Facile Synthesis of PEGylated Gold Nanoparticles for Enhanced Colorimetric Detection of Histamine

ACS Omega. 2024 Mar 13;9(12):14269-14278. doi: 10.1021/acsomega.3c10050. eCollection 2024 Mar 26.

Authors

Jahor Omping¹, Romnick Unabia¹, Renzo Luis Reazo¹, Melbagrace Lapening¹, Ryan Lumod^{1

2}, Archie Ruda¹, Rolen Brian Rivera^{1

2}, Noel Lito Sayson^{1

2}, Felmer Latayada³, Rey Capangpangan⁴, Gerard Dumancas⁵, Roberto Malaluan^{1

6}, Arnold Lubguban^{1

6}, Gaudencio Petalcorin Jr⁷, Arnold Alguno^{1

2}

Affiliations

¹ Research Center for Energy Efficient Materials (RCEEM), Premier Research Institute of Science and Mathematics (PRISM), Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines.
² Department of Physics, Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines.
³ Department of Chemistry, Caraga State University, Butuan City 8600, Philippines.
⁴ Department of Physical Sciences and Mathematics, College of Marine and Allied Sciences, Mindanao State University at Naawan, Naawan 9023, Misamis Oriental, Philippines.
⁵ Department of Chemistry, Loyola Science Center, The University of Scranton, Scranton, Pennsylvania 18510, United States.
⁶ Center for Sustainable Polymers, MSU-Iligan Institute of Technology, Iligan City 9200, Philippines.
⁷ Department of Mathematics and Statistics, Mindanao State University-Iligan Institute of Technology, 9200 Iligan City, Philippines.

Abstract

Histamine is among the biogenic amines that are formed during the microbial decarboxylation of amino acids in various food products, posing a significant threat to both food safety and human health. Herein, we present a one-step synthesis of PEGylated gold nanoparticles (PEG-AuNPs) for rapid, simple, and cost-effective colorimetric histamine detection. PEG-AuNPs' surface plasmon resonance (SPR) range at 520-530 nm with a hydrodynamic size distribution of 20-40 nm. Fourier transform infrared (FT-IR) spectra confirmed the reduction of AuNPs at 1645 cm^-1 along with the other observed peaks at 2870, 1350, and 1100 cm^-1 as a strong evidence for the presence of PEG. Upon the addition of histamine to the PEG-AuNP solution, transmission electron microscopy (TEM) highlighted the aggregation of nanoparticles. In addition, red shifting and a decrease in the absorbance of the SPR peak along with the appearance of an additional peak at ∼690 nm was observed in the PEG-AuNP absorption spectra in the presence of histamine. Increasing the PEG concentration in the gold colloids leads to the formation of a protective barrier around the surface of nanoparticles, which influences the colloidal stability by impeding the aggregation of PEG-AuNPs upon histamine addition. The minimum colorimetric response of PEG-AuNPs to histamine concentration is 30 ppm, as assessed by the naked eye. The absorption ratio (A₆₉₀/A₅₂₆) showed a linear dynamic range from 20 to 100 ppm with a limit of detection of 9.357 μM. Additionally, the assay demonstrates a commendable selectivity toward histamine analyte.