The effects of TiO2, ZnO, IONs and Al2O3 metallic nanoparticles on the CYP1A1 and NBN transcripts in rat liver

Walaa A Moselhy; Marwa A Ibrahim; Ahlam G Khalifa; El-Shaymaa El-Nahass; Nour El-Houda Y Hassan

doi:10.1093/toxres/tfae034

The effects of TiO2, ZnO, IONs and Al2O3 metallic nanoparticles on the CYP1A1 and NBN transcripts in rat liver

Toxicol Res (Camb). 2024 Mar 28;13(2):tfae034. doi: 10.1093/toxres/tfae034. eCollection 2024 Apr.

Authors

Walaa A Moselhy¹, Marwa A Ibrahim², Ahlam G Khalifa¹, El-Shaymaa El-Nahass³, Nour El-Houda Y Hassan¹

Affiliations

¹ Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
² Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Cairo 12211, Egypt.
³ Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.

PMID: 38559758
PMCID: PMC10980790 (available on 2025-03-28)
DOI: 10.1093/toxres/tfae034

Abstract

Introduction: Metal oxide nanoparticles are currently used widely in many aspects of human and animal life with broad prospects for biomedical purposes. The present work was carried out to investigate the effects of orally administrated TiO2NPs, ZnONPs, IONs and Al₂O₃NPs on the mRNA expression level of CYP 1A1 and NBN in the rat liver.

Materials and methods: Four groups of male Albino rats were given their respective treatment orally for 60 days in a dose of 1/20 of the LD50 TiO2NPs (600 mg/Kg b.wt/day), ZnONPs (340 mg/Kg b.wt/day), IONs (200 mg/kg b.wt/day) and Al₂O₃NPs (100 mg/Kg b.wt/day) and a fifth group served as a control group.

Rresults: The mRNA level of CYP 1A1 and NBN showed up-regulation in all the NPs-treated groups relative to the control group. ZnONPs group recorded the highest expression level while the TiO2NPs group showed the lowest expression level transcript. Conclusion:The toxic effects produced by these nanoparticles were more pronounced in the case of zinc oxide, followed by aluminum oxide, iron oxide nanoparticles and titanium dioxide, respectively.

Keywords: Al2O3NPs; CYP1A1; IONs; Liver; NBN; TiO2NPs; ZnONPs.