Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)

Rahul Rajendran; Rahul Krishnan; Myung-Joo Oh

doi:10.1016/j.jviromet.2024.114922

Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)

J Virol Methods. 2024 Mar 29:327:114922. doi: 10.1016/j.jviromet.2024.114922. Online ahead of print.

Authors

Rahul Rajendran¹, Rahul Krishnan², Myung-Joo Oh³

Affiliations

¹ Department of Aqualife Medicine, Chonnam National University, Yeosu 50626, Republic of Korea.
² Department of Aquatic Animal Health Management, Kerala University of Fisheries and Ocean Studies, Kerala 682506, India.
³ Department of Aqualife Medicine, Chonnam National University, Yeosu 50626, Republic of Korea. Electronic address: ohmj@jnu.ac.kr.

PMID: 38556175
DOI: 10.1016/j.jviromet.2024.114922

Abstract

A 2D primary gill cell culture system of the sevenband grouper (Hyporthodus septemfasciatus) was established to validate the pathogenesis of nervous necrosis virus (NNV) as observed in previous studies. This system, developed using the double-seeded insert (DSI) technique, yielded confluent cell layers. Upon challenge with NNV in a setup containing both autoclaved salt water and L15 media in the apical compartment, viral replication akin to that anticipated based on previous studies was observed. Consequently, we advocate for the utilization of primary gill cell culture as a viable alternative to conventional methodologies for investigating host pathogen interactions.

Keywords: 2D primary cell culture; Gill epithelium; NNV; Sevenband grouper.