Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells

Abstract

Lysosomal acid lipase (LAL) is a key enzyme in the metabolic pathway of neutral lipids, whose deficiency (LAL-D) induces the differentiation of myeloid lineage cells into myeloid-derived suppressor cells (MDSCs), which promotes tumor growth and metastasis. This protocol provides detailed procedures for assessment of various LAL biochemical and physiological activities in Ly6G⁺ and CD11c⁺ MDSCs, including isolation of Ly6G⁺ and CD11c⁺ cells from the bone marrow and blood of mice, assays of LAL-D-induced cellular metabolic and mitochondrial activities, assessment of LAL-D-induced pathogenic immunosuppressive activity and tumor stimulatory activity. Pharmacological inhibition of the LAL activity was also described in both murine myeloid cells and human white blood cells.

Keywords: CD11c cells; Immunosuppression; Ly6G cells; Lysosomal acid lipase; Myeloid-derived suppressor cells; Tumor growth.