Fabrication of a 96-electrode array using carbon dioxide laser ablation

Supatinee Kongkaew; Yudtapum Thipwimonmas; Mareeyam Hayeeabu; Warakorn Limbut

doi:10.1016/j.talanta.2024.125912

Fabrication of a 96-electrode array using carbon dioxide laser ablation

Talanta. 2024 Mar 13:274:125912. doi: 10.1016/j.talanta.2024.125912. Online ahead of print.

Authors

Supatinee Kongkaew¹, Yudtapum Thipwimonmas², Mareeyam Hayeeabu², Warakorn Limbut³

Affiliations

¹ Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Forensic Science Innovation and Service Center, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
² Forensic Science Innovation and Service Center, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.
³ Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Forensic Science Innovation and Service Center, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand. Electronic address: warakorn.l@psu.ac.th.

PMID: 38547843
DOI: 10.1016/j.talanta.2024.125912

Abstract

The 96 laser-induced multigraphene electrode (96L-MGE) integrated microwell plate (96 L-MGE-MP) is described. Each cell includes separate working, auxiliary, and reference electrodes, and the array sits on a poly-methyl methacrylate (PMMA) well. The 96 electrochemical cells were fabricated by laser ablation of polyimide adhesive tape, which created laser-induced graphene electrodes (L-GE). The microwell was produced using laser ablation of the PMMA sheet as well. The morphology and electrochemical characterization of L-GE were controlled by tuning the laser processing. L-GE fabricated at laser power-laser speed ratios of 0.008-0.02 W s mm^-¹displayed good electrochemical behaviors. Under the optimal condition of L-GE fabrication, the measured L-GE surface roughness was 475.47 nm. The 96 L-MGE can be fabricated in 24.2 min and is compatible with various analytes. 10 benchmark redox compounds were shown as electrocatalytic examples. The performance of each analyte was investigated by voltammetry. As proof of concept, 96 L-MGE-MP was connected to a 96× connector for multichannel detection. The RSD of the 96 L-MGE-MPwas below 5.3%, which demonstrated good fabrication reproducibility.

Keywords: 96-electrode array; Custom microwell plate; Laser-induced graphene; Polyimide.