Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Diagn Microbiol Infect Dis. 2024 Jun;109(2):116233. doi: 10.1016/j.diagmicrobio.2024.116233. Epub 2024 Feb 28.

Abstract

Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

Keywords: Carriage; Enrichment; VRE; Vancomycin-resistant enterococci; vanB.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins* / genetics
  • Gram-Positive Bacterial Infections* / diagnosis
  • Gram-Positive Bacterial Infections* / microbiology
  • Humans
  • Polymerase Chain Reaction* / methods
  • Rectum / microbiology
  • Sensitivity and Specificity*
  • Vancomycin-Resistant Enterococci* / drug effects
  • Vancomycin-Resistant Enterococci* / genetics
  • Vancomycin-Resistant Enterococci* / isolation & purification

Substances

  • VanB protein, Enterococcus
  • Bacterial Proteins