Fas ligand regulate nerve injury and repair by affecting AKT, β-catenin, and NF-κB pathways

Yiyue Zhou; Yi Yao; Yumei Feng; Zhiyuan Qiu; Shixian Luo; Xinyu Shi; Dandan Gu; Maorong Jiang; Min Cai; Dengbing Yao

doi:10.1016/j.ibneur.2024.02.008

Fas ligand regulate nerve injury and repair by affecting AKT, β-catenin, and NF-κB pathways

IBRO Neurosci Rep. 2024 Mar 6:16:455-467. doi: 10.1016/j.ibneur.2024.02.008. eCollection 2024 Jun.

Authors

Yiyue Zhou¹, Yi Yao², Yumei Feng¹, Zhiyuan Qiu¹, Shixian Luo¹, Xinyu Shi³, Dandan Gu¹, Maorong Jiang¹, Min Cai³, Dengbing Yao¹

Affiliations

¹ School of Life Sciences, Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, China.
² Nantong University Xinglin College, Nantong, Jiangsu Province, China.
³ Medical School of Nantong University, 19 Qixiu Road, Nantong, Jiangsu 226001, China.

Abstract

Objective: To investigate the regulatory effect of Fas-L on the repair and regeneration of peripheral extension injury in rats.

Methods: This study aimed to explore the effects of Fas-L on apoptosis and axonal regeneration of dorsal root ganglion (DRG) cells in rat peripheral nerve repair and regeneration by using several relevant experimental techniques from the injured nerve animal model, cell biology, and molecular biology.

Results: The expression level of Fas-L in DRG tissues was significantly down-regulated after sciatic nerve injury. Interference with Fas-L can significantly promote the regeneration of DRG neuronal axons and inhibit apoptosis, while the overexpression of Fas-L is contrary to it. Moreover, Fas-L may play a role in the regulation of DRG function and the repair and regeneration of peripheral nerves in Sprague Dawley (SD) rats by affecting several signaling pathways, such as p-AKT/AKT, β-catenin, and NF-κB.

Conclusion: Fas-L may have a certain effect on the repair and regeneration of peripheral nerve injury in SD rats, which may provide an experimental basis and a new theoretical basis for the functional reconstruction of peripheral nerves.

Significance statement: The expression level of Fas-L in DRG tissues was significantly down-regulated after sciatic nerve injury. Fas-L can significantly promote the regeneration of DRG neuronal axons and inhibit apoptosis. Fas-L may play a role in the regulation of DRG function and the repair and regeneration of peripheral nerves in SD rats by affecting several signaling pathways, such as p-AKT/AKT, β-catenin, and NF-κB. Fas-L may have a certain effect on the repair and regeneration of peripheral nerve injury in SD rats, which may provide an experimental basis and a new theoretical basis for the functional reconstruction of peripheral nerves.

Keywords: Apoptosis; DRG neurons; Fas-L; Nerve degeneration; Nerve injury; Nerve regeneration; Peripheral nerves.