Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria

Abstract

Polycationic photosensitizers (PS) are not susceptible to aggregation in solutions, but their high local concentrations in Gram-negative bacteria can be sufficient for aggregation and reduced effectiveness of antibacterial photodynamic treatment. By measuring fluorescence spectra and kinetics we were able to evaluate the degree of aggregation of polycationic PS ZnPcChol₈in Gram-negative bacteria E.coliK12 TG1. Binding of ZnPcChol₈toE.coliK12 TG1 leads to an appearance of groups of molecules with shorter PS fluorescence lifetime, a decrease in fluorescence intensity and a shift in the fluorescence spectral maximum. However, we evaluated that about 88% of the fluorescing PS molecules in the bacteria were in an unaggregated state, which indicates only a small reduction in the generation of reactive oxygen species.

Keywords: aggregation; antibacterial photodynamic treatment; bacteria; fluorescence lifetime; photosensitizer; polycationic.