The conversion of chemically modified biomass into more valuable chemicals has recently gained significant attention from industry. In this study, we investigate the adsorption of fructose and its conversion into two trioses, glyceraldehyde (GLA) and dihydroxyacetone (DHA), on metal-organic frameworks using density functional theory calculations. The reaction mechanism proceeds through two main steps: first, the opening of the fructose ring; second, the retro-aldol fragmentation, which is favored over intramolecular hydrogen shifts. The substitution of a tetravalent metal in the metal-organic framework leads to different adsorption strengths in the order Hf-NU-1000 > Zr-NU-1000 > Ti-NU-1000. The catalytic activities of Hf-NU-1000 and Zr-NU-1000 are found to be similar. Both are more active than Ti-NU1000, corresponding to their relative Lewis acidity. It was found that functionalization of the organic linkers of the Hf-NU-1000 MOF does not improve its catalytic activity. The catalytic activity follows the order Hf-MOF-808 > Hf-NU-1000 > Hf-UIO-66 when based on either the overall activation energy or the turnover frequency (TOF).