Simulation and experimental study on the influence of lamina on nanoneedle penetration into the cell nucleus

Jie Zou; Bei Peng; Na Fan; Yang Liu

doi:10.1007/s10237-024-01836-4

Simulation and experimental study on the influence of lamina on nanoneedle penetration into the cell nucleus

Biomech Model Mechanobiol. 2024 Mar 25. doi: 10.1007/s10237-024-01836-4. Online ahead of print.

Authors

Jie Zou¹, Bei Peng¹, Na Fan², Yang Liu³

Affiliations

¹ School of Mechatronics Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, China.
² School of Mechatronics Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, China. na_fan@uestc.edu.cn.
³ College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China. liuyang_leon@scu.edu.cn.

PMID: 38526703
DOI: 10.1007/s10237-024-01836-4

Abstract

We have developed a finite element model to simulate the penetration of nanoneedles into the cellular nucleus. It is found that the nuclear lamina, the primary supporting structure of the nuclear membrane, plays a crucial role in maintaining the integrity of the nuclear envelope and enhancing stress concentration in the nuclear membrane. Notably, nuclear lamina A exhibits a more pronounced effect compared to nuclear lamina B. Subsequently, we further conducted experiments by controlling the time of osteopontin (OPN) treatment to modify the nuclear lamina density, and the results showed that an increase in nuclear lamina density enhances the probability of nanoneedle penetration into the nuclear membrane. Through employing both simulation and experimental techniques, we have gathered compelling evidence indicating that an augmented density of nuclear lamina A can enhance the penetration of nanoneedles into the nuclear membrane.

Keywords: Cell Nucleus; Lamina; Mechanics; Nanoneedle; Penetration.