Comparative cytogenetics of microsatellite distribution in two tetra fishes Astyanax bimaculatus (Linnaeus, 1758) and Psalidodon scabripinnis (Jenyns, 1842)

Rodrigo Petry Corrêa de Sousa; Ivanete de Oliveira Furo; Gláucia Caroline Silva-Oliveira; Rosigleyse Corrêa de Sousa-Felix; Carla Denise Bessa-Brito; Raynara Costa Mello; Iracilda Sampaio; Roberto Ferreira Artoni; Edivaldo Herculano Corrêa de Oliveira; Marcelo Vallinoto

doi:10.7717/peerj.16924

Comparative cytogenetics of microsatellite distribution in two tetra fishes Astyanax bimaculatus (Linnaeus, 1758) and Psalidodon scabripinnis (Jenyns, 1842)

PeerJ. 2024 Mar 20:12:e16924. doi: 10.7717/peerj.16924. eCollection 2024.

Affiliations

¹ Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil.
² Universidade Federal Rural da Amazônia, Parauapebas, Pará, Brazil.
³ Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
⁴ Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, Paraná, Brazil.
⁵ Seção do Meio Ambiente, Instituto Evandro Chagas, Ananindeua, Pará, Brazil.
⁶ Instituto de Ciências Naturais e Exatas, Universidade Federal do Pará, Belém, Pará, Brazil.
⁷ Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal.

Abstract

Background: The main cytogenetic studies of the Characidae family comprise the genera Astyanax and Psalidodon involving the use of repetitive DNA probes. However, for the microsatellite classes, studies are still scarce and the function of these sequences in the genome of these individuals is still not understood. Thus, we aimed to analyze and compare the distribution of microsatellite sequences in the species Astyanax bimaculatus and Psalidodon scabripinnis.

Methods: We collected biopsies from the fins of A. bimaculatus and P. scabripinnis to perform cell culture, followed by chromosome extraction, and mapped the distribution of 14 microsatellites by FISH in both species.

Results and discussion: The diploid number observed for both species was 2n = 50, with an acrocentric B microchromosome in A. bimaculatus and a metacentric B chromosome in P. scabripinnis. Regarding FISH, 11 probes hybridized in the karyotype of A. bimaculatus mainly in centromeric regions, and 13 probes hybridized in P. scabripinnis, mainly in telomeric regions, in addition to a large accumulation of microsatellite hybridization on its B chromosome.

Conclusion: Comparative FISH mapping of 14 microsatellite motifs revealed different patterns of distribution both in autosomes and supernumerary chromosomes of A. bimaculatus and P. scabripinnis, suggesting independent evolutionary processes in each of these species, representing excellent data on chromosome rearrangements and cytotaxonomy.

Keywords: B Chromosome; Characidae; Chromosomal evolution; Genetic diversity; Repetitive sequences.

MeSH terms

Animals
Centromere
Characidae* / genetics
Cytogenetics
Karyotyping
Microsatellite Repeats / genetics

Grants and funding

This research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) through of the productivity grant (303889/2022-5) and the research project (407536/2021-3). Financial support was also made available to us by the Pro-Reitoria de Pesquisa e Pós-Graduação of the Universidade Federal do Pará. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.