Imaging of perivascular adipose tissue in cardiometabolic diseases by Raman spectroscopy: Towards single-cell analysis

Ewa Stanek; Krzysztof Czamara

doi:10.1016/j.bbalip.2024.159484

Imaging of perivascular adipose tissue in cardiometabolic diseases by Raman spectroscopy: Towards single-cell analysis

Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Mar 21;1869(5):159484. doi: 10.1016/j.bbalip.2024.159484. Online ahead of print.

Authors

Ewa Stanek¹, Krzysztof Czamara²

Affiliations

¹ Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, Poland; Jagiellonian University, Doctoral School of Exact and Natural Sciences, 11 Lojasiewicza Str., 30-348 Krakow, Poland.
² Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, Poland. Electronic address: krzysztof.czamara@uj.edu.pl.

PMID: 38521491
DOI: 10.1016/j.bbalip.2024.159484

Abstract

Perivascular adipose tissue (PVAT) has emerged as a dynamic organ influencing vascular function and cardiovascular health. In this brief review, an overview of the recent research in the investigation of PVAT is presented, ranging from in vivo studies to single-cell methodologies, in particular those based on Raman spectroscopy. The strengths and limitations of each, emphasizing their contributions to the current understanding of PVAT biology were discussed. Ultimately, the integration of these diverse methodologies promises to uncover new therapeutic targets and diagnostic biomarkers, including those emerging from simple Raman spectroscopy-based measurements of alterations in lipid unsaturation degree, invariably associated with PVAT dysfunction.

Keywords: Adipogenesis; Bioimaging; Obesity; Perivascular adipose tissue; Raman spectroscopy; Single-cell analysis.

Publication types

Review