A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus

Arch Virol. 2024 Mar 23;169(4):82. doi: 10.1007/s00705-024-06003-9.

Abstract

Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses.

MeSH terms

  • Alphacoronavirus*
  • Animals
  • Coronavirus Infections* / diagnosis
  • Coronavirus Infections* / epidemiology
  • Coronavirus Infections* / veterinary
  • Deltacoronavirus*
  • Diarrhea / diagnosis
  • Diarrhea / veterinary
  • Porcine epidemic diarrhea virus* / genetics
  • Sensitivity and Specificity
  • Swine
  • Swine Diseases*

Supplementary concepts

  • Swine acute diarrhea syndrome coronavirus
  • Porcine coronavirus HKU15