Production, optimization and characterization of partially purified anti-mycotic compound from marine soil derived streptomycetes originating at unexplored region of Bay of Bengal, India

Ramasamy Vijayakumar; Suresh S S Raja; Chinnasamy Muthukumar; Ponmurugan Karuppiah; Annamalai Panneerselvam; Jothi Ramalingam Rajabathar; Nooruddin Thajuddin; Ramamoorthy Ayyamperumal

doi:10.1016/j.envres.2024.118698

Production, optimization and characterization of partially purified anti-mycotic compound from marine soil derived streptomycetes originating at unexplored region of Bay of Bengal, India

Environ Res. 2024 Mar 20;251(Pt 2):118698. doi: 10.1016/j.envres.2024.118698. Online ahead of print.

Authors

Ramasamy Vijayakumar¹, Suresh S S Raja², Chinnasamy Muthukumar³, Ponmurugan Karuppiah⁴, Annamalai Panneerselvam⁵, Jothi Ramalingam Rajabathar⁶, Nooruddin Thajuddin⁷, Ramamoorthy Ayyamperumal⁸

Affiliations

¹ Department of Microbiology, Bharathidasan University, Palkalaiperur, Tiruchirappalli, 620 024, Tamil Nadu, India. Electronic address: rvijayakumar1978@bdu.ac.in.
² Department of Microbiology, Bharathidasan University, Palkalaiperur, Tiruchirappalli, 620 024, Tamil Nadu, India.
³ Department of Botany, National College (Autonomous), Tiruchirappalli, 620 001, Tamil Nadu, India.
⁴ Department of Botany and Microbiology, College of Science, King Saud University, Post Box: 2455, Riyadh, Saudi Arabia.
⁵ Post Graduate and Research Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, 613 503, Tamil Nadu, India.
⁶ Department of Chemistry, College of Science, King Saud University, Post Box: 2455, Riyadh 11451, Saudi Arabia. Electronic address: jrajabathar@ksu.edu.sa.
⁷ Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620 024, India; Crescent Global Outreach Mission, B S Abdur Rahman Crescent Institute of Science and Technology (Deemed to be University), Vandalur, Chennai - 600 048, India.
⁸ College of Earth Sciences, Lanzhou University, Lanzhou, 730000, People's Republic of China; Department of Biomaterials, Saveetha Dental College and Hospitals, SIMATS, Saveetha University, Chennai, 600077, India. Electronic address: ramamoorthy@lzu.ed.cn.

PMID: 38518906
DOI: 10.1016/j.envres.2024.118698

Abstract

Sixty-eight morphologically distinct isolates of marine actinomycetes were derived from seashore, mangrove, and saltpan ecosystems located between the Palk Strait and Gulf of Mannar region, Bay of Bengal, Tamilnadu. Twenty-five (36.8%) isolates exhibited anti-mycotic activity against Candida albicans and Cryptococcus neoformans in preliminary screening, and 4 isolates with prominent activity were identified and designated at the genus level as Streptomyces sp. VPTS3-I, Streptomyces sp. VPTS3-2, Streptomyces sp. VPTSA1-4 and Streptomyces sp. VPTSA1-8. All the potential antagonistic isolates were further characterized with phenotypic and genotypic properties including 16S rRNA gene sequencing and identified species level as Streptomyces afghaniensis VPTS3-1, S. matensis VPTS3-2, S. tuirus VPTSA1-4 and S. griseus VPTSA1-8. In addition, the active fractions from the potential antagonistic streptomycetes were extracted with organic solvents by shake flask culture method and the anti-mycotic efficacies were evaluated. The optimization parameters for the production of the anti-mycotic compound were found to be pH between 7 and 8, the temperature at 30ᵒC, the salinity of 2%, incubation of 9 days, and starch and KNO₃ as the suitable carbon and nitrogen sources respectively in starch casein medium.

Keywords: 16S rDNA sequencing; Anti-mycotic activity; Compound characterization; In vitro optimization; Marine streptomycetes.