Minor ergosteroids and a 19-nor labdane-type diterpenoid with anti-inflammatory effects from Ganoderma lucidum

Dan Cai; Yun-Yun Liu; Xin-Ping Tang; Mei Zhang; Yong-Xian Cheng

doi:10.1016/j.phytochem.2024.114052

Minor ergosteroids and a 19-nor labdane-type diterpenoid with anti-inflammatory effects from Ganoderma lucidum

Phytochemistry. 2024 Mar 20:222:114052. doi: 10.1016/j.phytochem.2024.114052. Online ahead of print.

Authors

Dan Cai¹, Yun-Yun Liu¹, Xin-Ping Tang², Mei Zhang³, Yong-Xian Cheng⁴

Affiliations

¹ State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Institute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen, 518055, China.
² Institute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen, 518055, China.
³ State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address: zhangmei63@cdutcm.edu.cn.
⁴ State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Institute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen, 518055, China. Electronic address: yxcheng@szu.edu.cn.

PMID: 38518849
DOI: 10.1016/j.phytochem.2024.114052

Abstract

A chemical investigation on the fruiting bodies of Ganoderma lucidum led to the isolation and identification of five undescribed ergosteroids including two des-D-steroids (3 and 4) and one rare 6/6/7/5-fused carbon skeletal ergosterol (5) along with one 19-nor labdane-type diterpenoid (6). Their structures including their absolute configurations, were assigned by spectroscopic methods, ECD calculations, and X-ray diffraction analysis. In addition, the anti-inflammatory activities of all the isolates were evaluated. The results indicated that compound 1 can significantly down-regulate the protein expression of iNOS and COX-2 at 20 μM in LPS- stimulated RAW264.7 cells.

Keywords: Anti-inflammatory; Baoslingzhines K–P; Diterpenoid; Ganoderma lucidum; Labdane-type; Steroids.