Surfactants from detergents, when inadequately treated in sewage treatment plants, are carried away into estuaries, resulting in the contamination of aquatic environments. It is thus necessary to develop rapid and efficient techniques that are capable of effectively monitoring these pollutants. In this context, for the first time in the literature, this study reports the development and application of a digital image-based (DIB) method for the in situ quantification of the anionic surfactant linear alkylbenzene sulfonate (LAS) in water bodies using a smartphone. The DIB method is a highly effective modern detection method based on methylene blue, which is employed as a modified alternative technique to the spectrophotometric method and commonly used in environmental studies; in the DIB method, the images of interest are obtained using a smartphone and the analyses are carried out using free software Color grab. The results obtained from the application of the DIB method showed that the method possesses high precision and accuracy, with a linear calibration curve in the range of 0.15 to 4.5 mg L-1, R2 = 0.993, a limit of detection of 6.0 μg L-1, and recovery rates ranging from 82.7% to 114%. The efficacy of the proposed method was evaluated by comparing its results with those of spectrophotometry (used as a reference method) through the analysis of environmental samples obtained from the Capibaribe River Estuary using methylene blue. No statistically significant differences were observed between the results obtained from the DIB and the spectrophotometric method. The innovative method proposed in this study is fast, economical and environmentally friendly; the technique involves the use of only a few microliters of samples and generates little waste compared to spectrophotometry. In addition, the proposed method is applicable for in situ analyses, allowing real-time monitoring of LAS in different types of aquatic environments.