Preparation of synthetic micro- and nano plastics for method validation studies

Blaž Hrovat; Emilia Uurasjärvi; Mirka Viitala; Ana Franco Del Pino; Mika Mänttäri; Nikolaos Papamatthaiakis; Antti Haapala; Kai Peiponen; Matthieu Roussey; Arto Koistinen

doi:10.1016/j.scitotenv.2024.171821

Preparation of synthetic micro- and nano plastics for method validation studies

Sci Total Environ. 2024 May 15:925:171821. doi: 10.1016/j.scitotenv.2024.171821. Epub 2024 Mar 19.

Authors

Affiliations

¹ University of Eastern Finland, Department of Technical Physics, P.O. Box 1627, 70211 Kuopio, Finland. Electronic address: blaz.hrovat@uef.fi.
² University of Eastern Finland, Department of Technical Physics, P.O. Box 1627, 70211 Kuopio, Finland.
³ Lappeenranta-Lahti University of Technology LUT, Department of Separation Science, Sammonkatu 12, 50130 Mikkeli, Finland.
⁴ Lappeenranta-Lahti University of Technology LUT, Department of Separation Science, Sammonkatu 12, 50130 Mikkeli, Finland; University of Cádiz, Department of Environmental Technology, 11510 Puerto Real, Cádiz, Spain.
⁵ University of Eastern Finland, School of Forest Science, P.O. Box 111, 80101 Joensuu, Finland.
⁶ University of Eastern Finland, Department of Chemistry, P.O. Box 111, 80101 Joensuu, Finland; FSCN Research Centre, Mid Sweden University, SE-85170 Sundsvall, Sweden.
⁷ University of Eastern Finland, Center for Photonics Sciences, Department of Physics and Mathematics, P.O. Box 111, 80101 Joensuu, Finland.

PMID: 38513866
DOI: 10.1016/j.scitotenv.2024.171821

Abstract

Microplastic (MP) pollution is a persisting global problem. Accurate analysis is essential in quantifying the effects of microplastic pollution and develop novel technologies that reliably and reproducibly measure microplastic content in various samples. The most common methods for this are FTIR and Raman spectroscopy. Coloured, standardized beads are often used for method validation tests, which limits the conclusions to a very specific case rarely observed in the natural environment. This study focuses on the preparation of reference micro- and nanoplastics via cryogenic milling and shows their use for FTIR and Raman method validation studies. MPs can now be reproducibly milled from various plastics, offering the advantages of a better representation of MPs in real environment. Moreover, this study highlights issues with the current detection methods, up to now considered as the most reliable ones for MP detection and identification. Such issues, e.g. misidentification, will need to be addressed in the future. Additionally, milled MPs were used in experiments with commercial high-resolution imaging device, enabling a possible in-situ optical detection of microplastics. These experiments represent a step forward in understanding MPs in a water sample and provide a basis for a more accurate detection and identification directly from water, which would considerably reduce the time of analysis.

Keywords: FTIR spectroscopy; Imaging; Microplastics; Raman spectroscopy; Validation.