Aggregation-induced emission nanoparticles facilitating multicolor lateral flow immunoassay for rapid and simultaneous detection of aflatoxin B1 and zearalenone

Yi Zhang; Guoxin Chen; Xirui Chen; Xiaxia Wei; Xuan-Ang Shen; Hu Jiang; Xiaoyang Li; Yonghua Xiong; Xiaolin Huang

doi:10.1016/j.foodchem.2024.138997

Aggregation-induced emission nanoparticles facilitating multicolor lateral flow immunoassay for rapid and simultaneous detection of aflatoxin B1 and zearalenone

Food Chem. 2024 Jul 30:447:138997. doi: 10.1016/j.foodchem.2024.138997. Epub 2024 Mar 15.

Authors

Yi Zhang¹, Guoxin Chen¹, Xirui Chen¹, Xiaxia Wei¹, Xuan-Ang Shen¹, Hu Jiang², Xiaoyang Li¹, Yonghua Xiong², Xiaolin Huang³

Affiliations

¹ State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China; School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
² State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China; School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China.
³ State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China; School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China. Electronic address: xiaolin.huang@ncu.edu.cn.

PMID: 38513493
DOI: 10.1016/j.foodchem.2024.138997

Abstract

Herein we developed a multicolor lateral flow immunoassay (LFIA) test strip for rapid and simultaneous quantitative detection of aflatoxin B1 (AFB1) and zearalenone (ZEN). Three differently colored aggregation-induced emission nanoparticles (AIENPs) were designed as LFIA signal tags, with red and green AIENPs for targeting AFB1 and ZEN at the test line, and yellow AIENPs for indicating the validity of the test strip at the control (C) line. After surface functionalization with antibodies, the developed AIENP-based multicolor LFIA allows simultaneous and accurate quantification of AFB1 and ZEN using an independent C-line assisted ratiometric signal output strategy. The detection limits of AFB₁ and ZEN were 6.12 and 26 pg/mL, respectively. The potential of this method for real-world applications was well demonstrated in corn and wheat. Overall, this multicolor LFIA shows great potential for field screening of multiple mycotoxins and can be extended to rapid and simultaneous monitoring of other small molecule targets.

Keywords: Aflatoxin B1; Aggregation-induced emission nanoparticles; Lateral flow immunoassay; Multiplexing detection; Mycotoxins; Zearalenone.

MeSH terms

Aflatoxin B1 / analysis
Antibodies, Monoclonal
Food Contamination / analysis
Immunoassay / methods
Limit of Detection
Metal Nanoparticles*
Mycotoxins* / analysis
Zearalenone* / analysis

Substances

Zearalenone
Aflatoxin B1
Antibodies, Monoclonal
Mycotoxins