Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T Cells Using JC-1 Dye

Ankit Shah; Marina A Dobrovolskaia

doi:10.1007/978-1-0716-3786-9_16

Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T Cells Using JC-1 Dye

Methods Mol Biol. 2024:2789:153-159. doi: 10.1007/978-1-0716-3786-9_16.

Authors

Ankit Shah¹, Marina A Dobrovolskaia²

Affiliations

¹ Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
² Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. marina@mail.nih.gov.

PMID: 38507001
DOI: 10.1007/978-1-0716-3786-9_16

Abstract

Alterations in mitochondrial membrane potential are associated with the generation of reactive oxygen species and cell death. While eliminating cancer cells is beneficial for cancer therapy, cytotoxicity to healthy cells may limit the therapeutic applications of mitochondria-damaging nanoparticles. Due to the critical role mitochondria play in cell viability and function, it is important to detect such alterations when studying nanomaterials for therapeutic applications. The protocol described herein utilizes JC-1 dye to detect nanoparticle-mediated changes in mitochondrial membrane potential and is intended to support mechanistic immunotoxicology studies.

Keywords: Membrane potential; Mitochondria; Nanoparticles; Oxidative stress; T cells.

MeSH terms

Benzimidazoles*
Carbocyanines*
Membrane Potential, Mitochondrial
Nanoparticles*
Oxidative Stress
Reactive Oxygen Species / metabolism
T-Lymphocytes* / metabolism

Substances

5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine
Reactive Oxygen Species
Benzimidazoles
Carbocyanines