Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Abstract

The maternal decidua is a transient and dynamic tissue that functions as an immunoprivileged matrix related to nutritional and endocrine processes. The function of decidual cells is key to the success of embryo implantation and the maintenance of pregnancy with a positive maternal-fetal outcome. Therefore, establishing a method to optimize the isolation of primary decidual cells is essential. Our protocol described here provides a good yield of decidual cells in an optimized time.

Keywords: Decidua; Isolation; Pregnancy; Primary cells; Vimentin.