A De Novo Whole Genome Assembly and Annotation of Parelaphostrongylus tenuis

Tyler J Garwood; Jessie E Richards; Marissa G Macchietto; Richard W Gerhold; Stephen A Kania; John R Garbe; Nicholas M Fountain-Jones; Peter A Larsen; Tiffany M Wolf

doi:10.2478/jofnem-2024-0009

A De Novo Whole Genome Assembly and Annotation of Parelaphostrongylus tenuis

J Nematol. 2024 Mar 14;56(1):20240009. doi: 10.2478/jofnem-2024-0009. eCollection 2024 Mar.

Authors

Tyler J Garwood¹, Jessie E Richards², Marissa G Macchietto³, Richard W Gerhold², Stephen A Kania², John R Garbe⁴, Nicholas M Fountain-Jones⁵, Peter A Larsen⁶, Tiffany M Wolf¹

Affiliations

¹ Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota-Twin Cities, Room 495 Animal Science/Veterinary Medicine Building, 1988 Fitch Avenue, St. Paul, MN, 55108.
² Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee-Knoxville, 2407 River Drive, Knoxville, TN, 37996.
³ Minnesota Supercomputing Institute, University of Minnesota-Twin Cities, 117 Pleasant Street SE, 547 Walter Library, Minneapolis, MN, 55455.
⁴ Genomics Center, University of Minnesota-Twin Cities, 2231 6th Street SE, Minneapolis, MN, 55455.
⁵ School of Natural Sciences, University of Tasmania, Private Bag 37, Hobart, Tasmania, Australia 7001.
⁶ Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota-Twin Cities, Room 239B Veterinary Science Building, 1971 Commonwealth Avenue, Saint Paul, MN, 55108.

Abstract

Parelaphostrongylus tenuis causes ungulate morbidity and mortality in eastern and central North America, but no reference genome sequence exists to facilitate research. Here, we present a P. tenuis genome assembly and annotation, generated with PacBio and Illumina technologies. The assembly is 491 Mbp, with 7285 scaffolds and 185 kb N50.

Keywords: Annotation; Parelaphostrongylus tenuis; assembly; brainworm; genome; genomics; meningeal worm; reference.